Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4770426..4770928 Replicon chromosome
Accession NZ_CP053731
Organism Escherichia coli strain CP55_Sichuan

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HP431_RS22860 Protein ID WP_000767829.1
Coordinates 4770674..4770928 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HP431_RS22855 Protein ID WP_001259255.1
Coordinates 4770426..4770677 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HP431_RS22830 4765604..4766671 - 1068 WP_001563933.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
HP431_RS22835 4766671..4767741 - 1071 WP_001682999.1 histidinol-phosphate transaminase -
HP431_RS22840 4767738..4769042 - 1305 WP_042031741.1 histidinol dehydrogenase -
HP431_RS22845 4769048..4769947 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
HP431_RS22850 4770093..4770143 - 51 WP_001364200.1 his operon leader peptide -
HP431_RS22855 4770426..4770677 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HP431_RS22860 4770674..4770928 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HP431_RS22865 4771011..4771835 + 825 WP_000754749.1 SDR family oxidoreductase -
HP431_RS22870 4771881..4772810 + 930 WP_000803351.1 LysR family transcriptional regulator -
HP431_RS22875 4773025..4773087 + 63 WP_010723108.1 membrane protein YoeI -
HP431_RS22880 4773077..4774435 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
HP431_RS22885 4774614..4775672 + 1059 WP_000492315.1 transport protein YeeE -
HP431_RS22890 4775686..4775913 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T157639 WP_000767829.1 NZ_CP053731:4770674-4770928 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T157639 NZ_CP069439:c89307-89158 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT157639 WP_001259255.1 NZ_CP053731:4770426-4770677 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT157639 NZ_CP069439:89355-89411 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References