Detailed information of TA system TA157633

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2696435..2697037
• Replicon: chromosome
• Accession: NZ_CP053731
• Organism: Escherichia coli strain CP55_Sichuan

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: HP431_RS12920
• Protein ID: WP_000897305.1
• Coordinates: 2696726..2697037 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HP431_RS12915
• Protein ID: WP_000356397.1
• Coordinates: 2696435..2696725 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HP431_RS12890	2692360..2693262	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HP431_RS12895	2693259..2693894	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HP431_RS12900	2693891..2694820	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
HP431_RS12905	2695150..2695392	-	243	WP_001087409.1	hypothetical protein	-
HP431_RS12910	2695612..2695830	-	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
HP431_RS12915	2696435..2696725	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HP431_RS12920	2696726..2697037	-	312	WP_000897305.1	hypothetical protein	Toxin
HP431_RS12925	2697266..2698174	+	909	WP_001162704.1	alpha/beta hydrolase	-
HP431_RS12930	2698238..2699179	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HP431_RS12935	2699224..2699661	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HP431_RS12940	2699658..2700530	-	873	WP_000920762.1	virulence factor BrkB family protein	-
HP431_RS12945	2700524..2701123	-	600	WP_001295269.1	glucose-1-phosphatase	-
HP431_RS12950	2701222..2702007	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T157633 WP_000897305.1 NZ_CP053731:c2697037-2696726 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T157633 NZ_CP069438:c3723971-3723633 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGCTACCCGGATTACAACCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAAGAGAGCGAA
CTGATGCAGTCACTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAAGCAGGTGCAGGATTTTATTGGGGTGATTAG
TAGCAAAACGCCACGCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT157633 WP_000356397.1 NZ_CP053731:c2696725-2696435 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT157633 NZ_CP069438:3723966-3724042 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATGAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7