Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | DinJ-YafQ (relBE)/YafQ-RelB |
| Location | 1775629..1776170 | Replicon | chromosome |
| Accession | NZ_CP053731 | ||
| Organism | Escherichia coli strain CP55_Sichuan | ||
Toxin (Protein)
| Gene name | yafQ | Uniprot ID | U9XPG0 |
| Locus tag | HP431_RS08690 | Protein ID | WP_000615976.1 |
| Coordinates | 1775892..1776170 (+) | Length | 93 a.a. |
Antitoxin (Protein)
| Gene name | dinJ | Uniprot ID | S1EWQ0 |
| Locus tag | HP431_RS08685 | Protein ID | WP_000729704.1 |
| Coordinates | 1775629..1775889 (+) | Length | 87 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HP431_RS08660 | 1771412..1772197 | - | 786 | WP_000207575.1 | putative lateral flagellar export/assembly protein LafU | - |
| HP431_RS08665 | 1772142..1773881 | + | 1740 | WP_001296890.1 | flagellar biosynthesis protein FlhA | - |
| HP431_RS08670 | 1773986..1774264 | + | 279 | WP_000598760.1 | type II toxin-antitoxin system HicA family toxin | - |
| HP431_RS08675 | 1774257..1774613 | + | 357 | WP_001030483.1 | type II toxin-antitoxin system HicB family antitoxin | - |
| HP431_RS08680 | 1774670..1775443 | - | 774 | WP_000543895.1 | C40 family peptidase | - |
| HP431_RS08685 | 1775629..1775889 | + | 261 | WP_000729704.1 | type II toxin-antitoxin system antitoxin DinJ | Antitoxin |
| HP431_RS08690 | 1775892..1776170 | + | 279 | WP_000615976.1 | type II toxin-antitoxin system mRNA interferase toxin YafQ | Toxin |
| HP431_RS08695 | 1776326..1777066 | + | 741 | WP_001225679.1 | murein L,D-transpeptidase | - |
| HP431_RS08700 | 1777037..1777804 | - | 768 | WP_000333380.1 | class II glutamine amidotransferase | - |
| HP431_RS08705 | 1778010..1778588 | - | 579 | WP_000284050.1 | D-sedoheptulose 7-phosphate isomerase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | gmhA/lpcA | 1774257..1786956 | 12699 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 93 a.a. Molecular weight: 10800.59 Da Isoelectric Point: 10.0702
>T157628 WP_000615976.1 NZ_CP053731:1775892-1776170 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG
Download Length: 279 bp
>T157628 NZ_CP069438:c2664490-2664383 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAGCTGGCTGAACAAGCGGAAGTAA
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAGCTGGCTGAACAAGCGGAAGTAA
Antitoxin
Download Length: 87 a.a. Molecular weight: 9391.77 Da Isoelectric Point: 5.0758
>AT157628 WP_000729704.1 NZ_CP053731:1775629-1775889 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI
Download Length: 261 bp
>AT157628 NZ_CP069438:2664547-2664605 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCTC
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCTC
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | U9XPG0 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| PDB | 4ML0 | |
| AlphaFold DB | A0A0E0Y6Z6 |