Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3556626..3557128 Replicon chromosome
Accession NZ_CP053730
Organism Escherichia coli strain CP61_Sichuan

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HP432_RS18220 Protein ID WP_000767829.1
Coordinates 3556626..3556880 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HP432_RS18225 Protein ID WP_001259255.1
Coordinates 3556877..3557128 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HP432_RS18190 3551641..3551868 - 228 WP_000985280.1 sulfurtransferase TusA family protein -
HP432_RS18195 3551882..3552940 - 1059 WP_000492315.1 transport protein YeeE -
HP432_RS18200 3553119..3554477 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
HP432_RS18205 3554467..3554529 - 63 WP_010723108.1 membrane protein YoeI -
HP432_RS18210 3554744..3555673 - 930 WP_024190947.1 LysR family transcriptional regulator -
HP432_RS18215 3555719..3556543 - 825 WP_000754737.1 SDR family oxidoreductase -
HP432_RS18220 3556626..3556880 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HP432_RS18225 3556877..3557128 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HP432_RS18230 3557412..3557462 + 51 WP_001364200.1 his operon leader peptide -
HP432_RS18235 3557608..3558507 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
HP432_RS18240 3558513..3559817 + 1305 WP_021547023.1 histidinol dehydrogenase -
HP432_RS18245 3559814..3560884 + 1071 WP_126699561.1 histidinol-phosphate transaminase -
HP432_RS18250 3560884..3561951 + 1068 WP_172615787.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T157611 WP_000767829.1 NZ_CP053730:c3556880-3556626 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T157611 NZ_CP069391:703231-703581 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT157611 WP_001259255.1 NZ_CP053730:c3557128-3556877 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT157611 NZ_CP069391:702944-703225 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References