Detailed information of TA system TA157593

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1708088..1708782
• Replicon: chromosome
• Accession: NZ_CP053730
• Organism: Escherichia coli strain CP61_Sichuan

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: HP432_RS09030
• Protein ID: WP_001263493.1
• Coordinates: 1708384..1708782 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: HP432_RS09025
• Protein ID: WP_000554757.1
• Coordinates: 1708088..1708381 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HP432_RS09000	1703905..1704372	+	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-
HP432_RS09005	1704392..1705108	+	717	WP_000938723.1	FliA/WhiG family RNA polymerase sigma factor	-
HP432_RS09010	1705121..1705984	+	864	WP_001169518.1	flagellar motor stator protein MotA	-
HP432_RS09015	1705987..1706910	+	924	WP_172615698.1	putative lateral flagellar export/assembly protein LafU	-
HP432_RS09020	1706981..1708036	+	1056	WP_001226164.1	DNA polymerase IV	-
HP432_RS09025	1708088..1708381	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HP432_RS09030	1708384..1708782	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HP432_RS09035	1708792..1709244	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
HP432_RS09040	1709478..1709744	+	267	WP_001321003.1	hypothetical protein	-
HP432_RS09045	1709656..1710213	+	558	WP_001353768.1	peptide chain release factor H	-
HP432_RS09050	1710270..1711727	-	1458	WP_126699615.1	cytosol nonspecific dipeptidase	-
HP432_RS09055	1711988..1712446	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
HP432_RS09060	1712538..1713782	+	1245	WP_000189541.1	esterase FrsA	-
-	1713042..1713122	+	81	NuclAT_11	-	-
-	1713042..1713122	+	81	NuclAT_11	-	-
-	1713042..1713122	+	81	NuclAT_11	-	-
-	1713042..1713122	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T157593 WP_001263493.1 NZ_CP053730:1708384-1708782 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T157593 NZ_CP069386:c1793325-1793029 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT157593 WP_000554757.1 NZ_CP053730:1708088-1708381 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT157593 NZ_CP069386:c1793027-1792632 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6