Detailed information of TA system TA157584

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 53357..53626
• Replicon: plasmid pCP66-6-IncFII
• Accession: NZ_CP053725
• Organism: Escherichia coli strain CP66-6_Sichuan

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HP433_RS23995
• Protein ID: WP_001312861.1
• Coordinates: 53468..53626 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	53357..53422 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HP433_RS23955	48702..48890	+	189	WP_032146593.1	hypothetical protein	-
HP433_RS23960	48892..49098	+	207	WP_000275858.1	hypothetical protein	-
HP433_RS23965	49124..49663	+	540	WP_001825195.1	single-stranded DNA-binding protein	-
HP433_RS23970	49726..49959	+	234	WP_000005990.1	DUF905 family protein	-
HP433_RS23975	50025..51983	+	1959	WP_001825193.1	ParB/RepB/Spo0J family partition protein	-
HP433_RS23980	52038..52472	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HP433_RS23985	52469..53188	+	720	WP_013362833.1	plasmid SOS inhibition protein A	-
HP433_RS23990	53200..53388	-	189	WP_001299721.1	hypothetical protein	-
-	53200..53424	+	225	NuclAT_0	-	-
-	53200..53424	+	225	NuclAT_0	-	-
-	53200..53424	+	225	NuclAT_0	-	-
-	53200..53424	+	225	NuclAT_0	-	-
-	53357..53422	+	66	-	-	Antitoxin
HP433_RS23995	53468..53626	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
HP433_RS24000	54127..54315	+	189	WP_042111937.1	hypothetical protein	-
HP433_RS24005	54548..54835	+	288	WP_000107544.1	hypothetical protein	-
HP433_RS24010	54956..55777	+	822	WP_172615468.1	DUF945 domain-containing protein	-
HP433_RS24015	56074..56721	-	648	WP_031943493.1	transglycosylase SLT domain-containing protein	-
HP433_RS24020	56998..57381	+	384	WP_000124981.1	relaxosome protein TraM	-
HP433_RS24025	57572..58258	+	687	WP_000332484.1	PAS domain-containing protein	-
HP433_RS24030	58352..58579	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA12 / mph(A) / fosA3 / blaTEM-215 / mcr-3.5	-	1..74817	74817

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T157584 WP_001312861.1 NZ_CP053725:53468-53626 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T157584 NZ_CP069386:c275951-275664 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT157584 NZ_CP053725:53357-53422 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin