Detailed information of TA system TA157578

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 69122..69548
• Replicon: plasmid pCP66-6-IncFIC
• Accession: NZ_CP053724
• Organism: Escherichia coli strain CP66-6_Sichuan

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HP433_RS23495
• Protein ID: WP_001312861.1
• Coordinates: 69122..69280 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	69324..69548 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HP433_RS23465	64663..66477	-	1815	Protein_76	type IV secretion system protein TraC	-
HP433_RS23470	66637..66858	-	222	WP_001278692.1	conjugal transfer protein TraR	-
HP433_RS23475	66993..67508	-	516	WP_000724019.1	type IV conjugative transfer system lipoprotein TraV	-
HP433_RS23480	67505..67873	-	369	WP_077254276.1	conjugal transfer protein TrbD	-
HP433_RS23485	67812..68399	-	588	WP_000002772.1	conjugal transfer protein TraP	-
HP433_RS23490	68389..68904	-	516	Protein_81	conjugal transfer protein TrbI	-
HP433_RS23495	69122..69280	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	69324..69548	-	225	NuclAT_0	-	Antitoxin
-	69324..69548	-	225	NuclAT_0	-	Antitoxin
-	69324..69548	-	225	NuclAT_0	-	Antitoxin
-	69324..69548	-	225	NuclAT_0	-	Antitoxin
HP433_RS23500	69360..69548	+	189	WP_001299721.1	hypothetical protein	-
HP433_RS23505	69560..70279	-	720	WP_013362833.1	plasmid SOS inhibition protein A	-
HP433_RS23510	70276..70683	-	408	WP_172615466.1	conjugation system SOS inhibitor PsiB	-
HP433_RS23515	70649..70915	-	267	Protein_86	class I SAM-dependent methyltransferase	-
HP433_RS23520	70963..72324	-	1362	WP_000170715.1	DUF3560 domain-containing protein	-
HP433_RS23525	72376..72606	-	231	WP_000218642.1	hypothetical protein	-
HP433_RS23530	73510..73887	+	378	WP_000457136.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(M) / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / floR / sul2 / tet(A) / aph(3')-Ia / sul3 / blaTEM-215 / lnu(F) / aac(3)-IId	-	1..99734	99734

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T157578 WP_001312861.1 NZ_CP053724:c69280-69122 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T157578 NZ_CP069381:c4593471-4593160 [Salmonella enterica]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT157578 NZ_CP053724:c69548-69324 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin