Detailed information of TA system TA157574

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 59213..59467
• Replicon: plasmid pCP66-6-IncFIC
• Accession: NZ_CP053724
• Organism: Escherichia coli strain CP66-6_Sichuan

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: HP433_RS23440
• Protein ID: WP_001351576.1
• Coordinates: 59213..59419 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	59406..59467 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HP433_RS23405	55234..55734	+	501	Protein_64	Tn3 family transposase	-
HP433_RS23410	55768..55956	+	189	Protein_65	transposase	-
HP433_RS23415	56031..56318	-	288	WP_000222766.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HP433_RS23420	56315..56566	-	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
HP433_RS23425	57532..58389	-	858	WP_039002220.1	incFII family plasmid replication initiator RepA	-
HP433_RS23430	58382..58456	-	75	WP_061357229.1	RepA leader peptide Tap	-
HP433_RS23435	58688..58945	-	258	WP_001541895.1	replication regulatory protein RepA	-
HP433_RS23440	59213..59419	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	59406..59467	+	62	NuclAT_1	-	Antitoxin
-	59406..59467	+	62	NuclAT_1	-	Antitoxin
-	59406..59467	+	62	NuclAT_1	-	Antitoxin
-	59406..59467	+	62	NuclAT_1	-	Antitoxin
HP433_RS23445	59809..60021	-	213	WP_001541896.1	ANR family transcriptional regulator	-
HP433_RS23450	60156..60566	-	411	Protein_73	fertility inhibition protein FinO	-
HP433_RS23455	60569..63454	-	2886	Protein_74	conjugative transfer relaxase/helicase TraI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(M) / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / floR / sul2 / tet(A) / aph(3')-Ia / sul3 / blaTEM-215 / lnu(F) / aac(3)-IId	-	1..99734	99734

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T157574 WP_001351576.1 NZ_CP053724:c59419-59213 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T157574 NZ_CP069381:3442357-3442575 [Salmonella enterica]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT157574 NZ_CP053724:59406-59467 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin