Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4617337..4617939 Replicon chromosome
Accession NZ_CP053723
Organism Escherichia coli strain CP66-6_Sichuan

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag HP433_RS22135 Protein ID WP_000897305.1
Coordinates 4617628..4617939 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HP433_RS22130 Protein ID WP_000356397.1
Coordinates 4617337..4617627 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HP433_RS22105 4613282..4614184 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
HP433_RS22110 4614181..4614816 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
HP433_RS22115 4614813..4615742 + 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
HP433_RS22120 4616072..4616314 - 243 WP_001087409.1 hypothetical protein -
HP433_RS22125 4616533..4616751 - 219 WP_001295676.1 ribbon-helix-helix domain-containing protein -
HP433_RS22130 4617337..4617627 - 291 WP_000356397.1 helix-turn-helix domain-containing protein Antitoxin
HP433_RS22135 4617628..4617939 - 312 WP_000897305.1 hypothetical protein Toxin
HP433_RS22140 4618168..4619076 + 909 WP_001162704.1 alpha/beta hydrolase -
HP433_RS22145 4619140..4620081 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
HP433_RS22150 4620126..4620563 - 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -
HP433_RS22155 4620560..4621432 - 873 WP_000920762.1 virulence factor BrkB family protein -
HP433_RS22160 4621426..4622025 - 600 WP_001295269.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4608872..4617939 9067


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T157572 WP_000897305.1 NZ_CP053723:c4617939-4617628 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T157572 NZ_CP069381:2001655-2001758 [Salmonella enterica]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT157572 WP_000356397.1 NZ_CP053723:c4617627-4617337 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT157572 NZ_CP069381:c2001760-2001615 [Salmonella enterica]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References