Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1946568..1947070 Replicon chromosome
Accession NZ_CP053723
Organism Escherichia coli strain CP66-6_Sichuan

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HP433_RS09345 Protein ID WP_000767829.1
Coordinates 1946816..1947070 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HP433_RS09340 Protein ID WP_001259255.1
Coordinates 1946568..1946819 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HP433_RS09315 1941746..1942813 - 1068 WP_000080130.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
HP433_RS09320 1942813..1943883 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
HP433_RS09325 1943880..1945184 - 1305 WP_001542955.1 histidinol dehydrogenase -
HP433_RS09330 1945190..1946089 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
HP433_RS09335 1946235..1946285 - 51 WP_001364200.1 his operon leader peptide -
HP433_RS09340 1946568..1946819 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HP433_RS09345 1946816..1947070 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HP433_RS09350 1947153..1947977 + 825 WP_000754758.1 SDR family oxidoreductase -
HP433_RS09355 1948023..1948952 + 930 WP_000803351.1 LysR family transcriptional regulator -
HP433_RS09360 1949167..1949229 + 63 WP_010723108.1 membrane protein YoeI -
HP433_RS09365 1949219..1950577 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
HP433_RS09370 1950756..1951814 + 1059 WP_000492346.1 transport protein YeeE -
HP433_RS09375 1951828..1952055 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T157555 WP_000767829.1 NZ_CP053723:1946816-1947070 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T157555 NZ_CP069355:c106284-105025 [Microvirga sp. VF16]
ATGACGCACCTGCTCGATGTCTGGTTCGGCGATACAAAGGCGGGCATCCTCGCTCAGGACGAGGCAGGTGCTCTGAGCTA
CGCCTATGATCCCAACTATCTCGCCGGACGAGAGCCGCGCTCCATCTCCTTTTCCATGCCATTGCAGGAGGCGCCTTACG
CCGACCGTGTGGTTCGTCCCTTTTTCTCAGGCCTGCTACCGGATGAGGGCGCGCGGCAGCGCCTCGCAGGTGCGCTCGGC
ATTTCTGCTGGCAATGCCTTTGGGCTCCTGGAAGTGATCGGTGGTGAATGCGCTGGGGCCTTGTCTCTCTATCGGGCCGG
CGAGGCGCCGCCATCGTCGGATGATGATGGCGTCGAACTGCTGAACCCGGCGCGGCTCGAAGAAATCCTTGGCAAGCTGC
GGGAGCGTCCTTTGCTCGGCGGTGAGGAAGGTGTCCGGCTGTCCTTAGCCGGCGCGCAGGACAAGCTTGCAGTCTGCGTG
GAAGGCGACGATATCGGCCTCGCCAAAGGCGGCCGACCAACTACGCATATCCTCAAGCCCTTCATTCAGGCGCTCGACGG
CACGGTGGAGAACGAACTGTTCTGCCTGCACCTTGCAGCGCGCCTGAAACTTCCGGTGCCGGCCGTCGAGATGCGCCGCA
CTGGCGCGATATCCTTCCTGATCATCGAACGCTACGACCGCTCGCCGCACAAGGGCGGAACGATCACACGCTTGCACCAG
GAAGATTTCTGTCAGGCTTTGAGCGTGCCGCCGGAGCTCAAATATGAAGAGGAAGGCGGACCGGGGACAGAGAAGGCACT
GGACCTGATTGGCCGCGCCACCGCGCGTCCGGCGGCAGACCGTCTGCGCTTCCTTCGTATGCTGATCTTCCATTACCTTG
TCGGCAACGCCGACGCTCACGGAAAGAATTACGCTTTGCTCTATCGCGGCAAGGTTCCTGACCTTGCGCCGCTATATGAT
GTCGTTTGCACGGCTGCATATCCGCGGCTCGCGAAGAAGCTCGCTATGGCGATCGGCGGGCGCTCGGTTCCAGACACGAT
ACAGTTGCAACACTGGCTCACGCTCGTCCCGGAGACGCGCAGCGCTCAGCGCCTTCTGGTCAAGGACATAGCCGAACTGG
CAGGGCGCATCAGAGGCGAGGCAGACAAGCTTCTGGCAGAATTTGAGGGGGCGGGCGTTACCCATGAAATTCTTACAGTT
GTCCGTCGTATCATTGAGGCTCGCGCGGCGCATTTGTTGAGAATTGCGACGCCTAATTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT157555 WP_001259255.1 NZ_CP053723:1946568-1946819 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT157555 NZ_CP069355:c106508-106281 [Microvirga sp. VF16]
ATGCAAACGGTTTTAGACAGCAAAAGCTTTGGTCTCATAATCCGCCAGGAACGGAGGTCGCAGAAACTCACACAGGAACA
GCTCGCCGGGCTTACCGGCGTGGGCGTTCGGTTTGTGCGCGAGTTGGAGGCGGGAAAGGAGAGCTGTCAGATCGGACGCG
CTCTTCAGGTGGCGGCCGCTCTAGGCCTTAGCGTCTCCGTCGGAAGCCGCCTAGAGAGTGCGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References