Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 620066..620865 Replicon chromosome
Accession NZ_CP053723
Organism Escherichia coli strain CP66-6_Sichuan

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag HP433_RS02970 Protein ID WP_000347266.1
Coordinates 620066..620530 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HP433_RS02975 Protein ID WP_001307405.1
Coordinates 620530..620865 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HP433_RS02940 615067..615501 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
HP433_RS02945 615519..616397 - 879 WP_001315856.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
HP433_RS02950 616387..617166 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
HP433_RS02955 617177..617650 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HP433_RS02960 617673..618953 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
HP433_RS02965 619202..620011 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HP433_RS02970 620066..620530 - 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HP433_RS02975 620530..620865 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HP433_RS02980 621014..622585 - 1572 WP_106464846.1 galactarate dehydratase -
HP433_RS02985 622960..624294 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
HP433_RS02990 624310..625080 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 620066..632800 12734


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T157550 WP_000347266.1 NZ_CP053723:c620530-620066 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T157550 NZ_CP069352:5281703-5281957 [Pseudomonas granadensis]
ATGAAAATCCAGTTCACGCCCACGGGCTGGGAAGACTATTTGTGGTTTCAACAGAACGACAAGGCCGGACTCAAGCGCAT
CAATCTCCTGATCAAAGCGATCAAGCGCGAACCATTTGAGGGCGTCGGAAAGCCAGAGGCGCTCAAGCACAACATGAGCG
GCCTCTGGTCTCGCCGGATAACTGCGGAGCATCGTCTGGTTTACATGATTGAGGACGGCGAGGTGTGCGTCATCAGCTGC
AGATACCACTATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT157550 WP_001307405.1 NZ_CP053723:c620865-620530 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT157550 NZ_CP069352:5281452-5281706 [Pseudomonas granadensis]
ATGCAAACCATCAACTACACAACCGCCCGTGCGCACTTGGCCGAGACGATGGACCGTGTCAATGAAGACCGTGCGCCTCT
ACTGGTTACCCGGCAGAAAGGGGAACCTGTTGTGATGATGTCCCTGTCCGATTACAACGCGCTAGAAGAAACAGCCTATT
TGCTGCGCTCACCTGCCAACGCCGAGCGCTTGATGAAGTCCATCAACGAGCTGCGCGCCGGTAAAGCCAAAGCCAGGCAA
CTGATTGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References