Detailed information of TA system TA157545

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 96654..96908
• Replicon: plasmid pCP131-IncFIB
• Accession: NZ_CP053722
• Organism: Escherichia coli strain CP131_Sichuan

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: HP434_RS23630
• Protein ID: WP_001351576.1
• Coordinates: 96702..96908 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	96654..96715 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HP434_RS23615	94604..95350	+	747	WP_172615439.1	conjugal transfer pilus acetylase TraX	-
HP434_RS23620	95405..95965	+	561	WP_001541898.1	fertility inhibition protein FinO	-
HP434_RS23625	96100..96312	+	213	WP_001541896.1	ANR family transcriptional regulator	-
-	96654..96715	-	62	NuclAT_0	-	Antitoxin
-	96654..96715	-	62	NuclAT_0	-	Antitoxin
-	96654..96715	-	62	NuclAT_0	-	Antitoxin
-	96654..96715	-	62	NuclAT_0	-	Antitoxin
HP434_RS23630	96702..96908	+	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
HP434_RS23635	97192..97449	+	258	WP_001541895.1	replication regulatory protein RepA	-
HP434_RS23640	97681..97755	+	75	WP_061357229.1	RepA leader peptide Tap	-
HP434_RS23645	97748..98605	+	858	WP_061583826.1	incFII family plasmid replication initiator RepA	-
HP434_RS23650	98961..99638	+	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
HP434_RS23655	99638..99985	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
HP434_RS23660	100005..101576	+	1572	WP_000381395.1	IS66 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / sul3 / mph(A) / floR / blaTEM-1B / tet(M) / aadA2 / dfrA12	-	1..121655	121655

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T157545 WP_001351576.1 NZ_CP053722:96702-96908 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T157545 NZ_CP069352:c2478890-2478582 [Pseudomonas granadensis]
ATGAGTCAGGCTTTAACCAATTTCGACATGGCATCACTGCTGGACAGCGACGAAGCCATCAGCGAATACCTGTCTCAGGT
ACTCGCAGATGGAGATAACGAAGAGTTTCTTCGCGCAATCGGTCATGTGCTCAAGGCCCGTGGAATGACGCAGATAGCCA
AAAGCTCAGGCATGGGCCGCGAGAGTCTGTACAAGGCGTTCGCGCCGGGTGCAAAACCCCGCTTCGATACAGTTCTGAAA
GTGATTCATGCCCTCGGCATGGACCTCTGCGCACAACCGGGGCATGTGATCAATCACTCGGCTTCCTGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT157545 NZ_CP053722:c96715-96654 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin