Detailed information of TA system TA157508

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 19389..19906
• Replicon: plasmid unnamed8
• Accession: NZ_CP053715
• Organism: Lichenicola cladoniae strain PAMC 26569

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HN018_RS28325
• Protein ID: WP_171837324.1
• Coordinates: 19712..19906 (-)
• Length: 65 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HN018_RS28320
• Protein ID: WP_171837323.1
• Coordinates: 19389..19715 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HN018_RS28290 (HN018_28305)	14526..15233	+	708	WP_171837347.1	hypothetical protein	-
HN018_RS28295 (HN018_28310)	15230..15865	+	636	WP_171837348.1	hypothetical protein	-
HN018_RS28300 (HN018_28315)	15937..16452	+	516	WP_171837349.1	hypothetical protein	-
HN018_RS28305 (HN018_28320)	16571..17092	-	522	WP_171837350.1	hypothetical protein	-
HN018_RS28310 (HN018_28325)	17270..18316	+	1047	WP_172443453.1	IS30 family transposase	-
HN018_RS28315 (HN018_28330)	18602..18979	-	378	WP_239479507.1	helix-turn-helix domain-containing protein	-
HN018_RS29035 (HN018_28335)	18993..19349	-	357	WP_239479508.1	hypothetical protein	-
HN018_RS28320 (HN018_28340)	19389..19715	-	327	WP_171837323.1	helix-turn-helix transcriptional regulator	Antitoxin
HN018_RS28325 (HN018_28345)	19712..19906	-	195	WP_171837324.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HN018_RS28330 (HN018_28350)	20050..21282	+	1233	WP_171837325.1	AAA family ATPase	-
HN018_RS28335 (HN018_28355)	21378..21848	+	471	WP_239479509.1	hypothetical protein	-
HN018_RS28340 (HN018_28360)	21907..22089	-	183	WP_204259814.1	hypothetical protein	-
HN018_RS28345 (HN018_28365)	22355..22981	-	627	WP_171837327.1	helix-turn-helix domain-containing protein	-
HN018_RS28350 (HN018_28370)	23313..23936	+	624	WP_171837328.1	ParA family protein	-
HN018_RS28355 (HN018_28375)	23933..24184	+	252	WP_171837329.1	hypothetical protein	-
HN018_RS28360 (HN018_28380)	24253..24831	-	579	WP_171837351.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..28800	28800

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-59)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-86)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7512.89 Da
• Isoelectric Point: 12.2192

>T157508 WP_171837324.1 NZ_CP053715:c19906-19712 [Lichenicola cladoniae]
MQFRFRCYRTPEMRFSGRDGIARAIYVTAIGRRVIVVHAFVKKTQKTLRATLALAERRAQEITR

Nucleotide

• Length: 195 bp

>T157508 NZ_CP069332:c143237-142956 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 11690.61 Da
• Isoelectric Point: 9.0821

>AT157508 WP_171837323.1 NZ_CP053715:c19715-19389 [Lichenicola cladoniae]
MTSLAKLRQKLLTDPEVKAEYDRLGPIFAVVGEMIEARQAAGLTQAEIAARMGTTQSVVARLETARHMPTFDLVARYAAA
IDRRLDIRLVPNGAPDGLSSGLMRPACP

Nucleotide

• Length: 327 bp

>AT157508 NZ_CP069332:c143461-143234 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA