Detailed information of TA system TA157403

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3857163..3857760
• Replicon: chromosome
• Accession: NZ_CP053666
• Organism: Klebsiella pneumoniae strain kpn-241

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: HPK15_RS18915
• Protein ID: WP_004893639.1
• Coordinates: 3857443..3857760 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: HPK15_RS18910
• Protein ID: WP_004142561.1
• Coordinates: 3857163..3857450 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPK15_RS18880 (3853370)	3853370..3853618	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
HPK15_RS18885 (3853636)	3853636..3853977	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
HPK15_RS18890 (3854008)	3854008..3855123	-	1116	WP_074180844.1	MBL fold metallo-hydrolase	-
HPK15_RS18895 (3855303)	3855303..3855884	+	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
HPK15_RS18900 (3855884)	3855884..3856252	+	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
HPK15_RS18905 (3856372)	3856372..3857025	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
HPK15_RS18910 (3857163)	3857163..3857450	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
HPK15_RS18915 (3857443)	3857443..3857760	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HPK15_RS18920 (3857945)	3857945..3858988	-	1044	WP_023279204.1	DUF2157 domain-containing protein	-
HPK15_RS18925 (3859658)	3859658..3860524	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
HPK15_RS18930 (3860633)	3860633..3862060	+	1428	WP_004176980.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T157403 WP_004893639.1 NZ_CP053666:c3857760-3857443 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T157403 NZ_CP069215:981905-982084 [Staphylococcus epidermidis]
ATGGCGAACAAAAAAGATTCCAAATTAAATTATCATGAAGAAGAAAATGCTATGGTTACCGACTTAGATGATTTAAAAGA
ATTAGGAAAAGAAATGGAACAGATTTCACAGGAAAATGATGAAGAAAAATTAAATCAATCTCATGATAACGAAGTTCGTT
CAGATTTAAAAAAACAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT157403 WP_004142561.1 NZ_CP053666:c3857450-3857163 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT157403 NZ_CP069215:981282-981881 [Staphylococcus epidermidis]
ATGGCAATGAATTTTAAAGTATTTGATAATAGTGAAAAAGTAGCAGAATATACTGCAGATATTCTACGTAAGCAGTTTAA
TAATAATCCAACTACTATTGCAGGATTTCATTTGAGCAAAGAACATGCACCAGTGTTTGATGAACTTAAGAAAAATGTAG
AAAATCATACAGTTGATTTCAGTCAAATAAATATTTTAGATTATGATGATAATCATTCTTATTATGAGGCTTTGGGTGTG
CCAACAGGCCAAATTTATTCCATTTCTTACGAAAATGATGCAATTGACTTTATCTCAGATAAGATTAAGACTAAAGAAAA
TAAAGGCAAACTGACAATGCAAGTCCTTACGATTGATGAAAATGGTAAACTTGACATTAGCGTTCGTCAAGGTTTGATGG
AAGCTAGAGAAATTTTCTTAGTTATTACTGGTGCTAATAAACGCGATATGGTAGAAAAACTTTACCGTGAAAATGGTAAG
ACTAGTTTTGAACCATCGGATTTAAAAGCACACCGTATGGTCAATGTTATCTTAGATAAAGAAGCGGCTGCAGGCTTACC
TGAAGACGTAAAAGAGTACTTCACTGCACGTTTTGCATAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1