Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 353737..354383 Replicon chromosome
Accession NZ_CP053666
Organism Klebsiella pneumoniae strain kpn-241

Toxin (Protein)


Gene name higB Uniprot ID A0A486LFK7
Locus tag HPK15_RS01630 Protein ID WP_023301313.1
Coordinates 353737..354084 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag HPK15_RS01635 Protein ID WP_002920557.1
Coordinates 354084..354383 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPK15_RS01620 (349663) 349663..351096 + 1434 WP_002920564.1 glycogen synthase GlgA -
HPK15_RS01625 (351114) 351114..353561 + 2448 WP_002920561.1 glycogen phosphorylase -
HPK15_RS01630 (353737) 353737..354084 + 348 WP_023301313.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HPK15_RS01635 (354084) 354084..354383 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
HPK15_RS01640 (354446) 354446..355954 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
HPK15_RS01645 (356159) 356159..356488 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
HPK15_RS01650 (356539) 356539..357369 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
HPK15_RS01655 (357419) 357419..358177 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13506.49 Da        Isoelectric Point: 6.2327

>T157391 WP_023301313.1 NZ_CP053666:353737-354084 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKETITMADREFSQHLTKER

Download         Length: 348 bp

>T157391 NZ_CP069203:c4783938-4783654 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCAGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT157391 WP_002920557.1 NZ_CP053666:354084-354383 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT157391 NZ_CP069203:c4784170-4783928 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A486LFK7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References