Detailed information of TA system TA157385

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 328870..329109
• Replicon: plasmid unnamed
• Accession: NZ_CP053663
• Organism: Escherichia coli strain eco-241

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: HPK14_RS27655
• Protein ID: WP_023144756.1
• Coordinates: 328975..329109 (+)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	328870..328930 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPK14_RS27635 (326400)	326400..327146	+	747	WP_000205725.1	conjugal transfer pilus acetylase TraX	-
HPK14_RS27640 (327201)	327201..327761	+	561	WP_000139341.1	fertility inhibition protein FinO	-
HPK14_RS27645 (327892)	327892..328104	+	213	WP_013023861.1	hypothetical protein	-
HPK14_RS27650 (328617)	328617..328903	+	287	Protein_402	DUF2726 domain-containing protein	-
- (328870)	328870..328930	-	61	NuclAT_1	-	Antitoxin
- (328870)	328870..328930	-	61	NuclAT_1	-	Antitoxin
- (328870)	328870..328930	-	61	NuclAT_1	-	Antitoxin
- (328870)	328870..328930	-	61	NuclAT_1	-	Antitoxin
HPK14_RS27655 (328975)	328975..329109	+	135	WP_023144756.1	Hok/Gef family protein	Toxin
HPK14_RS27660 (329406)	329406..329660	+	255	WP_000083850.1	replication regulatory protein RepA	-
HPK14_RS27665 (329897)	329897..329971	+	75	WP_031943482.1	RepA leader peptide Tap	-
HPK14_RS27670 (329964)	329964..330821	+	858	WP_000130640.1	incFII family plasmid replication initiator RepA	-
HPK14_RS27675 (331761)	331761..332414	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
HPK14_RS27680 (332507)	332507..332764	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
HPK14_RS27685 (332697)	332697..333098	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
HPK14_RS27690 (333383)	333383..333523	+	141	Protein_410	DUF262 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaSFO-1 / blaTEM-1B / aac(3)-IId / dfrA12 / aadA2 / qacE / sul1 / blaNDM-1 / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / dfrA14 / tet(B)	senB	1..340412	340412
	-	flank	IS/Tn	-	-	333899..334276	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T157385 WP_023144756.1 NZ_CP053663:328975-329109 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T157385 NZ_CP069203:1495481-1495897 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATTATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACCGACATCCGGGTGGCGCTGCACCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCGGGCGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTGCCTGGACTGACACTGG
AAGACTGGGTTATTTAG

Antitoxin

Nucleotide

• Length: 61 bp

>AT157385 NZ_CP053663:c328930-328870 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin