Detailed information of TA system TA157382

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 289524..289793
• Replicon: plasmid unnamed
• Accession: NZ_CP053663
• Organism: Escherichia coli strain eco-241

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: HPK14_RS27425
• Protein ID: WP_001372321.1
• Coordinates: 289668..289793 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	289524..289589 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPK14_RS27380	284543..284818	+	276	WP_071780182.1	hypothetical protein	-
HPK14_RS27385	285059..285265	+	207	WP_000547975.1	hypothetical protein	-
HPK14_RS27390	285291..285830	+	540	WP_000290841.1	single-stranded DNA-binding protein	-
HPK14_RS27395	285893..286126	+	234	WP_000005990.1	DUF905 family protein	-
HPK14_RS27400	286192..288150	+	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-
HPK14_RS27405	288205..288639	+	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
HPK14_RS27410	288636..289398	+	763	Protein_354	plasmid SOS inhibition protein A	-
HPK14_RS27415	289367..289555	-	189	WP_001299721.1	hypothetical protein	-
-	289367..289591	+	225	NuclAT_0	-	-
-	289367..289591	+	225	NuclAT_0	-	-
-	289367..289591	+	225	NuclAT_0	-	-
-	289367..289591	+	225	NuclAT_0	-	-
-	289524..289589	+	66	-	-	Antitoxin
HPK14_RS27420	289577..289726	+	150	Protein_356	plasmid maintenance protein Mok	-
HPK14_RS27425	289668..289793	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
HPK14_RS27430	290013..290243	+	231	WP_001426396.1	hypothetical protein	-
HPK14_RS27435	290241..290414	-	174	Protein_359	hypothetical protein	-
HPK14_RS27440	290484..290690	+	207	WP_000275859.1	hypothetical protein	-
HPK14_RS27445	290715..291002	+	288	WP_000107535.1	hypothetical protein	-
HPK14_RS27450	291121..291942	+	822	WP_001234469.1	DUF932 domain-containing protein	-
HPK14_RS27455	292239..292841	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
HPK14_RS27460	293172..293555	+	384	WP_001151566.1	conjugal transfer relaxosome DNA-binding protein TraM	-
HPK14_RS27465	293689..294366	+	678	WP_001348626.1	PAS domain-containing protein	-
HPK14_RS27470	294454..294681	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaSFO-1 / blaTEM-1B / aac(3)-IId / dfrA12 / aadA2 / qacE / sul1 / blaNDM-1 / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / dfrA14 / tet(B)	senB	1..340412	340412

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T157382 WP_001372321.1 NZ_CP053663:289668-289793 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T157382 NZ_CP069203:331361-331729 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGACAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT157382 NZ_CP053663:289524-289589 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Antitoxin