Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 114470..115127 Replicon plasmid unnamed
Accession NZ_CP053663
Organism Escherichia coli strain eco-241

Toxin (Protein)


Gene name higB Uniprot ID U3PDC3
Locus tag HPK14_RS26350 Protein ID WP_000270043.1
Coordinates 114470..114820 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HPK14_RS26355 Protein ID WP_000124640.1
Coordinates 114825..115127 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPK14_RS26315 (110944) 110944..111441 - 498 WP_000062185.1 hypothetical protein -
HPK14_RS26320 (111444) 111444..111932 - 489 WP_001273096.1 DUF1643 domain-containing protein -
HPK14_RS26325 (112029) 112029..112364 + 336 WP_000683476.1 hypothetical protein -
HPK14_RS26330 (112379) 112379..112849 - 471 WP_001281821.1 hypothetical protein -
HPK14_RS26335 (112842) 112842..113213 - 372 WP_000516916.1 hypothetical protein -
HPK14_RS26340 (113224) 113224..113418 - 195 WP_000343597.1 hypothetical protein -
HPK14_RS26345 (113759) 113759..114307 - 549 WP_001061574.1 transcriptional regulator -
HPK14_RS26350 (114470) 114470..114820 + 351 WP_000270043.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HPK14_RS26355 (114825) 114825..115127 + 303 WP_000124640.1 XRE family transcriptional regulator Antitoxin
HPK14_RS26360 (115154) 115154..115447 - 294 WP_001239997.1 chromosome segregation protein ParM -
HPK14_RS26365 (115535) 115535..115807 - 273 WP_001043047.1 HU family DNA-binding protein -
HPK14_RS26370 (115865) 115865..116392 - 528 WP_001236377.1 thermonuclease family protein -
HPK14_RS26375 (116409) 116409..116597 - 189 WP_001270411.1 hypothetical protein -
HPK14_RS26380 (116623) 116623..117480 - 858 WP_001167032.1 hypothetical protein -
HPK14_RS26385 (117467) 117467..117697 - 231 WP_000972663.1 hypothetical protein -
HPK14_RS26390 (117697) 117697..118215 - 519 WP_000210756.1 nitrite reductase -
HPK14_RS26395 (118212) 118212..118658 - 447 WP_000919345.1 Fe3+-siderophore ABC transporter permease -
HPK14_RS26400 (118658) 118658..119017 - 360 WP_000422768.1 hypothetical protein -
HPK14_RS26405 (119074) 119074..119502 - 429 WP_072713905.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaSFO-1 / blaTEM-1B / aac(3)-IId / dfrA12 / aadA2 / qacE / sul1 / blaNDM-1 / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / dfrA14 / tet(B) senB 1..340412 340412


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-106)

Antitoxin

Cro/C1-type helix-turn-helix domain

(33-79)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13328.21 Da        Isoelectric Point: 5.2421

>T157379 WP_000270043.1 NZ_CP053663:114470-114820 [Escherichia coli]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Download         Length: 351 bp

>T157379 NZ_CP069198:4169311-4170384 [Pseudomonas aeruginosa]
GTGACTGAACGCGACTACAAAAACATCAACCTCAAACGATGCATTATCGAGGAAACGACTCATTGCTTCATCCGAGATAA
TGGCCTGAAGCTGCGATCCTATGGTGACAAGCCTGGGAGTTCCGGTCTTCGAGTAGTCTTCGGAAAAGCAGGGATTGAAG
ATGCCACGCTTGATATTTTTTTCACGAATGTCGGTGCGTCTACCCTGACCTACAAGGTGGGCAAGAACCAAGCTCTCGGC
CAGCAGCTAGCGGATGCTCTTTACGAAACAATCCACCCCGATGAATTCGTAACGATGAACCTTGGTATCAAAGGCGTCGA
TCTGGATGATGCCGAAGCCCTTATCAAGGAACTCACTGAGTCAGCCAATGCGGACATTGAGGTTGCTTCGCGCACAAGCG
CTGATCGCAAGCATATTTGGAAGCTCAAGTCTAAAAAGAACTTGGATGAGTTGACTGTCACTCAGCACGAGAACACCCAT
AAACTGCAGATCCAGGGTCGTCCTCTCAGCTGCTATCAGCAGTTGAGCTACCTGCTCACCGAGCTCCTAGAGATCAACGC
ACTCGAATGCATCCTGTTCAAAAAGGAAGAAAATCGCTCGGAGTTCGTTTGTGCCGAGGTCGCGGAAAGCGTACTCAAGG
AAAGGTTTGGCGACTGCTACGCCAACCTGCCACAGACCTCGCGTAGGCTGCTCCTGGCAAGCCTTTGCGTGAGGCTTGCC
AGCCCCGCGCTACCCGACTACTGCATGCTGGTTTATCCGGAACTCCGCACCCTTGAAGGCGCGATCAAACAGAAGCTTGC
AGAAAAAAACCTTTCAAATCAGGAAGAAAGCTTCGGTGGCTTCTTTACCAAGACCAATGGTCAGTTTTATCTGAAGCAGC
AATACCATGCTGATGTCGGCGATGCTAAACTACAGGGTCATCTGGACAACGCTTACACCTTCTTCAACAAGCAACGCCAC
GGATTGTTCCACATGGAAGAGATGCCATCCGTTAGTCGAATGATTAGCAACATCACCCAAGCTGTGTCATTGTGTGATGA
AGCATATGATCATATCAAGAATTTGTATAGCTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11006.73 Da        Isoelectric Point: 7.2036

>AT157379 WP_000124640.1 NZ_CP053663:114825-115127 [Escherichia coli]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Download         Length: 303 bp

>AT157379 NZ_CP069198:4170439-4170732 [Pseudomonas aeruginosa]
ATGACGGTAGTTACCGCTACCAGTTATGAGAACCCCTTATCTCGTCTTTCTATTATTGCTAGCGAGATGCGCAATACATC
TCACTCCAGCAAGGAGATAGTTCTATTCGATCTACTCTGCTCTAACGGAGAAGAGTGGAATCGTTTCGTCTCCATCAATT
ACAACGGCACCGACTTCGAAAAAAGCACCTGTAGCATTGTCTCCAAGTCCGACATCCCTACAGACCTCTTGGAAACACAA
ACTCGCTTCTTTCAAATCCATCCACAATATCTGCTTGATTCAGTTTTGAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7G8AFB9

Antitoxin

Source ID Structure

References