Detailed information of TA system TA157373

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-agrB/SymE(toxin)
• Location: 4458389..4458801
• Replicon: chromosome
• Accession: NZ_CP053662
• Organism: Escherichia coli strain eco-241

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: HPK14_RS21835
• Protein ID: WP_000132601.1
• Coordinates: 4458460..4458801 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	agrB
Locus tag	-
Coordinates	4458389..4458465 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPK14_RS21825 (4455252)	4455252..4456841	+	1590	WP_001063200.1	type I restriction-modification system methyltransferase	-
HPK14_RS21830 (4456838)	4456838..4458232	+	1395	WP_001272445.1	type I restriction-modification system specificity subunit	-
- (4458389)	4458389..4458465	-	77	NuclAT_12	-	Antitoxin
- (4458389)	4458389..4458465	-	77	NuclAT_12	-	Antitoxin
- (4458389)	4458389..4458465	-	77	NuclAT_12	-	Antitoxin
- (4458389)	4458389..4458465	-	77	NuclAT_12	-	Antitoxin
- (4458389)	4458389..4458465	-	77	NuclAT_13	-	Antitoxin
- (4458389)	4458389..4458465	-	77	NuclAT_13	-	Antitoxin
- (4458389)	4458389..4458465	-	77	NuclAT_13	-	Antitoxin
- (4458389)	4458389..4458465	-	77	NuclAT_13	-	Antitoxin
HPK14_RS21835 (4458460)	4458460..4458801	+	342	WP_000132601.1	endoribonuclease SymE	Toxin
HPK14_RS21840 (4458963)	4458963..4460342	+	1380	WP_000443951.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
HPK14_RS21845 (4460342)	4460342..4461388	+	1047	WP_147697023.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T157373 WP_000132601.1 NZ_CP053662:4458460-4458801 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T157373 NZ_CP069195:c1318476-1318198 [Vibrio diabolicus]
ATGATTTTATGGGAAGAAGAGTCACTTAATGATCGTGAAAAGATCTTCGAGTTTCTCTATGACTTCAACCCTGATGCGGC
AGAAAAAACTGACAACCTCATTGAAGCAAAAGTAGAAAACTTACTTGAACAACCTTTAATGGGTGTACAACGAGATGGCA
TCCGCGGACGATTGCTCATTATTCCTGAGATTTCGATGATTGTATCTTACTGGGTTGAGGGCGATATCATCCGAGTTATG
CGTGTACTACACCAGAAACAAAAATTCCCTACGGATTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT157373 NZ_CP053662:c4458465-4458389 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin