Detailed information of TA system TA157370

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4064547..4065197
• Replicon: chromosome
• Accession: NZ_CP053662
• Organism: Escherichia coli strain eco-241

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HPK14_RS19995
• Protein ID: WP_000263532.1
• Coordinates: 4064856..4065197 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: U9YXQ7
• Locus tag: HPK14_RS19990
• Protein ID: WP_000212552.1
• Coordinates: 4064547..4064846 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPK14_RS19965 (4059995)	4059995..4060306	-	312	WP_000415004.1	hypothetical protein	-
HPK14_RS19970 (4060311)	4060311..4060703	-	393	WP_000285322.1	flagellar export chaperone FliS	-
HPK14_RS19975 (4060726)	4060726..4062042	-	1317	WP_000609678.1	flagellar filament capping protein FliD	-
HPK14_RS19980 (4062247)	4062247..4063161	-	915	WP_000949083.1	lateral flagellin LafA	-
HPK14_RS19985 (4063647)	4063647..4064489	+	843	WP_000022366.1	winged helix-turn-helix domain-containing protein	-
HPK14_RS19990 (4064547)	4064547..4064846	-	300	WP_000212552.1	helix-turn-helix transcriptional regulator	Antitoxin
HPK14_RS19995 (4064856)	4064856..4065197	-	342	WP_000263532.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HPK14_RS20000 (4065273)	4065273..4066250	-	978	WP_001195938.1	hypothetical protein	-
HPK14_RS20005 (4066267)	4066267..4067196	-	930	WP_001266799.1	flagellar hook-associated protein FlgL	-
HPK14_RS20010 (4067211)	4067211..4068587	-	1377	WP_000367245.1	flagellar hook-associated protein FlgK	-
HPK14_RS20015 (4068763)	4068763..4069062	-	300	WP_000867281.1	rod-binding protein	-
HPK14_RS20020 (4069062)	4069062..4070162	-	1101	WP_001181876.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13031.86 Da
• Isoelectric Point: 6.4677

>T157370 WP_000263532.1 NZ_CP053662:c4065197-4064856 [Escherichia coli]
MWDVETTETFDNWFDAQTVALKEDLLAAMLILAEYGPQLGRPFADTVNDSQFSNMKELRVQHQGNPVRAFFAFDPARRGI
VLCAGDKTGLNEKKFYRDMIKLADSEYRKHLKK

Nucleotide

• Length: 342 bp

>T157370 NZ_CP069185:2724983-2725126 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGGAATCCTAGAGGCTGTTAAAGAAGACAATAAAAAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11004.86 Da
• Isoelectric Point: 9.7665

>AT157370 WP_000212552.1 NZ_CP053662:c4064846-4064547 [Escherichia coli]
MATLKELMAKQSPESQVRIAAKVEEMRQTIALHQLREELNISQTEMAAAMGIKQPTLAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPNGKRIAFQI

Nucleotide

• Length: 300 bp

>AT157370 NZ_CP069185:c2725320-2725058 [Enterococcus faecalis]
AAATAAAAATGCCCGAGCCTCTTATTTTTCTAAGGCTCGGGCATTTTTATTGATGGTCTTTTGAGGGTCGTTGTGCTATA
ATGGTAATGAAAAGAGAGGTATGCGCCAACATACCTCTCTAGTGTAGAGCCGTTTCAGACGGTAGCGATTTTTAGTGATT
TATAAAAAATAACCGTGCTCGCTAAAGTTGACGGTTATTTTTTATTGTCTTCTTTAACAGCCTCTAGGATTCCGAAGATT
AACGTTGCGATAAAAGAACCGAA

Structures

Antitoxin

• AlphaFold DB: U9YXQ7