Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2656339..2656901 Replicon chromosome
Accession NZ_CP053662
Organism Escherichia coli strain eco-241

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag HPK14_RS12910 Protein ID WP_000605676.1
Coordinates 2656339..2656617 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag HPK14_RS12915 Protein ID WP_000781370.1
Coordinates 2656617..2656901 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPK14_RS12885 (2651925) 2651925..2652356 - 432 WP_000152305.1 peroxiredoxin OsmC -
HPK14_RS12890 (2652701) 2652701..2652916 + 216 WP_000495766.1 biofilm-dependent modulation protein -
HPK14_RS12895 (2653018) 2653018..2653155 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
HPK14_RS12900 (2653312) 2653312..2655009 + 1698 WP_000433464.1 malate dehydrogenase -
HPK14_RS12905 (2655143) 2655143..2656153 + 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
HPK14_RS12910 (2656339) 2656339..2656617 + 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HPK14_RS12915 (2656617) 2656617..2656901 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
HPK14_RS12920 (2657129) 2657129..2657782 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
HPK14_RS12925 (2657775) 2657775..2658659 - 885 WP_001240565.1 formate dehydrogenase N subunit beta -
HPK14_RS12930 (2658672) 2658672..2661719 - 3048 WP_012602782.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T157365 WP_000605676.1 NZ_CP053662:2656339-2656617 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T157365 NZ_CP069178:336574-337416 [Bulleidia sp. zg-1006]
ATGAATAAAGCAAAATTAACAACTCTTTGCCATAAAGTCAGCAAAGAAGTCGGTCTTTCGTTTAATTCGGTTATGCTGTA
TTATTTTTTAGAAAGTATTTTGAAAAAATTAGCAGGAAGCAAATATAGTGAACGATTTATTTTTAAGGGCGACTTTTTGT
TATCAAGTGTAGTTGGAATTGATTCAAGAAGTACTGTAGATATAGACTTTCTTTTGAGAAACATGCAGTTATCAGAAGAA
AATATTGTTCAAATGCTGAACGAAACTTTAAAACCGGAAGAATCCGACGATATATTTTATGAATTGCAAAGTATTGTGCC
AATCAAAGAAGAAGATCAATATGGAGGGTTTCGTGCAAATATCTTATGTAAAATGGAAAATATAAGACAGATTGTACCAC
TTGACATTGCAACAGGAGATGTTATTACACCTCATCCGATAGATTATAAGTATGTAAGCTCTTTTGGAGAGGAAGAAATA
ATAATCAAGGCTTATCCGATAGAAACTATGCTTGCAGAAAAAATACAGACCATTTATGCAAGAGGATTTTTAAATAGTAG
AAGTAAGGATTATTACGATCTTTATATTATTTATAAATTAAAGGACAAAGATGTAAATGCAGAGATATTAAGGGAAGCTT
GTAGAAAAACTTTTCAGTATAGAAAAACAGAATTTGACATAGGTAAAATTATTGATTTGCTTGAAAACTTGAAAACAAAT
GAAGCTTTTTTGAAAAGATGGCAAGCCTATTCAAGAAAAAATATATATGCCAAGGATATAACCTTTGAAGAAGTATTGGA
TAATGGAATAAAAATGGTGAAAAAAATAAAGAATGAAGATTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT157365 WP_000781370.1 NZ_CP053662:2656617-2656901 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT157365 NZ_CP069178:335988-336581 [Bulleidia sp. zg-1006]
ATGAATTATGAAGATAAAATTGAACAACATCTAAAACAATCAGGTGGAATTATTACAACAGCATACTGTAAGGAAAATAA
TATACCGACGATATATCTCAGTCGACTATCGAAAGATGGGAGATTGTTGAAAATTAAAAAAGGAATTTATATGACAAAAG
ATGGAGATTATGATGAGTATTATTTCTTTCAATATCAGTATAAAAAGGCGATATTTTCTTATGAAACCGCTCTTTATCTG
CTTGGACAAACAGATAAAATTCCTTGGAATATCGATGTAACCGTTTATAACGGATATAAATTTAATGAAAAACCGAATGG
TTTCAATATTCATTATGTAAAAAAATCAATTTATGACTTAGGTGTAATCCAAAAACCTACAATGTTTGGAAATAAAGTTA
AGGTATATTCATATGAGAGGACACTCTGTGATTTTATTGCTCATAAAGAAGAAATGGATATAGAGGTTTATGTAAAACTT
ATTCGTTCGTATTCCTCTTATGAAGAAAGAGATGTTCATTCCCTTTATGATATTGCAATGAAAATGGGAATTGAAAATAA
AGTGAGAGAAGTTATGGAGGTGGTTTATGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References