Detailed information of TA system TA157353

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 741148..741841
• Replicon: chromosome
• Accession: NZ_CP053662
• Organism: Escherichia coli strain eco-241

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: HPK14_RS03615
• Protein ID: WP_000415584.1
• Coordinates: 741148..741444 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: HPK14_RS03620
• Protein ID: WP_000650107.1
• Coordinates: 741446..741841 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPK14_RS03580 (736236)	736236..736550	-	315	WP_000958598.1	putative quinol monooxygenase	-
HPK14_RS03585 (736581)	736581..737162	-	582	WP_000072421.1	NADPH:quinone oxidoreductase MdaB	-
HPK14_RS03590 (737481)	737481..737813	+	333	WP_000917684.1	DUF2645 family protein	-
HPK14_RS03595 (737859)	737859..739208	-	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
HPK14_RS03600 (739205)	739205..739864	-	660	WP_001221510.1	quorum sensing response regulator transcription factor QseB	-
HPK14_RS03605 (740016)	740016..740408	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
HPK14_RS03610 (740461)	740461..740943	+	483	WP_000183505.1	GyrI-like domain-containing protein	-
HPK14_RS03615 (741148)	741148..741444	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
HPK14_RS03620 (741446)	741446..741841	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
HPK14_RS03625 (741974)	741974..743581	+	1608	WP_001735743.1	ABC transporter substrate-binding protein	-
HPK14_RS03630 (743719)	743719..745977	+	2259	WP_001281841.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T157353 WP_000415584.1 NZ_CP053662:741148-741444 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T157353 NZ_CP069173:6163-6444 [Klebsiella pneumoniae]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTAAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT157353 WP_000650107.1 NZ_CP053662:741446-741841 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT157353 NZ_CP069173:5897-6166 [Klebsiella pneumoniae]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATTCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65