Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 678540..679267 Replicon chromosome
Accession NZ_CP053662
Organism Escherichia coli strain eco-241

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag HPK14_RS03315 Protein ID WP_000550189.1
Coordinates 678540..678854 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID D3GWQ0
Locus tag HPK14_RS03320 Protein ID WP_000560253.1
Coordinates 678851..679267 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPK14_RS03295 (674706) 674706..675692 - 987 WP_001377074.1 Gfo/Idh/MocA family oxidoreductase -
HPK14_RS03300 (675771) 675771..676454 - 684 WP_001183054.1 vancomycin high temperature exclusion protein -
HPK14_RS03305 (676531) 676531..677034 - 504 WP_001309773.1 M48 family metallopeptidase -
HPK14_RS03310 (677119) 677119..678255 + 1137 WP_000018677.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
HPK14_RS03315 (678540) 678540..678854 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
HPK14_RS03320 (678851) 678851..679267 + 417 WP_000560253.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
HPK14_RS03325 (679312) 679312..681330 - 2019 WP_000121438.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
HPK14_RS03330 (681501) 681501..683852 - 2352 WP_000695439.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T157352 WP_000550189.1 NZ_CP053662:678540-678854 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T157352 NZ_CP069172:c128177-128019 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14965.39 Da        Isoelectric Point: 4.4547

>AT157352 WP_000560253.1 NZ_CP053662:678851-679267 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYAQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT157352 NZ_CP069172:c128445-128221 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure
AlphaFold DB A0A829IXD3

References