157351

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 635492..636291 Replicon chromosome
Accession NZ_CP053662
Organism Escherichia coli strain eco-241

Toxin (Protein)


Gene name yhaV Uniprot ID B7NDB6
Locus tag HPK14_RS03090 Protein ID WP_000347269.1
Coordinates 635492..635956 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID V0YUB5
Locus tag HPK14_RS03095 Protein ID WP_001309780.1
Coordinates 635956..636291 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPK14_RS03060 (630493) 630493..630927 - 435 WP_000948818.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
HPK14_RS03065 (630945) 630945..631823 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
HPK14_RS03070 (631813) 631813..632592 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
HPK14_RS03075 (632603) 632603..633076 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HPK14_RS03080 (633099) 633099..634379 - 1281 WP_000681943.1 tagatose-bisphosphate aldolase subunit KbaZ -
HPK14_RS03085 (634628) 634628..635437 + 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
HPK14_RS03090 (635492) 635492..635956 - 465 WP_000347269.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HPK14_RS03095 (635956) 635956..636291 - 336 WP_001309780.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HPK14_RS03100 (636440) 636440..638011 - 1572 WP_001273738.1 galactarate dehydratase -
HPK14_RS03105 (638386) 638386..639720 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
HPK14_RS03110 (639736) 639736..640506 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 635492..646660 11168


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T157351 WP_000347269.1 NZ_CP053662:c635956-635492 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTREAEETH

Download         Length: 465 bp

>T157351 NZ_CP069172:c29357-29208 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12345.94 Da        Isoelectric Point: 4.8616

>AT157351 WP_001309780.1 NZ_CP053662:c636291-635956 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNT
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT157351 NZ_CP069172:29402-29461 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829IY86

Antitoxin

Source ID Structure
AlphaFold DB V0YUB5

References