Detailed information of TA system TA157301

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-SprF3/-
• Location: 2827852..2828159
• Replicon: chromosome
• Accession: NZ_CP053639
• Organism: Staphylococcus aureus strain 14732

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: Q2FWU9
• Locus tag: HA036_RS14325
• Protein ID: WP_011447039.1
• Coordinates: 2827983..2828159 (-)
• Length: 59 a.a.

Antitoxin (RNA)

Gene name	SprF3
Locus tag	-
Coordinates	2827852..2827991 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HA036_RS14285	2823418..2823597	+	180	WP_000669791.1	hypothetical protein	-
HA036_RS14290	2823908..2824168	+	261	WP_001791826.1	hypothetical protein	-
HA036_RS14295	2824221..2824571	-	351	WP_000702263.1	complement inhibitor SCIN-A	-
HA036_RS14300	2825082..2825417	-	336	Protein_2745	SH3 domain-containing protein	-
HA036_RS14305	2826068..2826559	-	492	WP_000920038.1	staphylokinase	-
HA036_RS14310	2826750..2827505	-	756	WP_000861038.1	CHAP domain-containing protein	-
HA036_RS14315	2827517..2827771	-	255	WP_000611512.1	phage holin	-
HA036_RS14320	2827823..2827930	+	108	WP_031762631.1	hypothetical protein	-
-	2827852..2827991	+	140	NuclAT_0	-	Antitoxin
-	2827852..2827991	+	140	NuclAT_0	-	Antitoxin
-	2827852..2827991	+	140	NuclAT_0	-	Antitoxin
-	2827852..2827991	+	140	NuclAT_0	-	Antitoxin
HA036_RS14325	2827983..2828159	-	177	WP_011447039.1	putative holin-like toxin	Toxin
HA036_RS14330	2828268..2829041	-	774	WP_000750412.1	staphylococcal enterotoxin type A	-
HA036_RS14335	2829414..2829788	-	375	WP_000340977.1	hypothetical protein	-
HA036_RS14340	2829844..2830131	-	288	WP_001262621.1	hypothetical protein	-
HA036_RS14345	2830177..2830329	-	153	WP_001000058.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	scn / sak / sea / hlb / groEL	2824221..2880337	56116

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (30-58)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6849.45 Da
• Isoelectric Point: 10.6777

>T157301 WP_011447039.1 NZ_CP053639:c2828159-2827983 [Staphylococcus aureus]
MDRWWLSEYKEVVPMVALLKSLERRRLMITISTMLQFGLFLIALIGLVIKLIELSNKK

Nucleotide

• Length: 177 bp

>T157301 NZ_CP069133:1503543-1503719 [Escherichia coli BW25113]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Nucleotide

• Length: 140 bp

>AT157301 NZ_CP053639:2827852-2827991 [Staphylococcus aureus]
ATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAGTGAGCCCGTTAAAAAGACGGTGGCTATTTTAGATTAAA
GATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGATGGTTATTTTTT

Structures

Toxin

• PDB: 7NS1

Antitoxin