Detailed information of TA system TA157208

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 142189..142610
• Replicon: plasmid F'Iq
• Accession: NZ_CP053608
• Organism: Escherichia coli strain NEB5-alpha_F'Iq

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: HO392_RS22850
• Protein ID: WP_001302184.1
• Coordinates: 142452..142610 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	142189..142387 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HO392_RS22825	138119..138658	+	540	WP_171831303.1	single-stranded DNA-binding protein	-
HO392_RS22830	138715..138948	+	234	WP_000005990.1	DUF905 family protein	-
HO392_RS22835	139014..140972	+	1959	WP_000117173.1	ParB/RepB/Spo0J family partition protein	-
HO392_RS22840	141027..141461	+	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
HO392_RS22845	141458..142177	+	720	WP_000116348.1	plasmid SOS inhibition protein A	-
-	142189..142387	+	199	NuclAT_0	-	Antitoxin
-	142189..142387	+	199	NuclAT_0	-	Antitoxin
-	142189..142387	+	199	NuclAT_0	-	Antitoxin
-	142189..142387	+	199	NuclAT_0	-	Antitoxin
HO392_RS22850	142452..142610	+	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
HO392_RS22855	142848..143207	-	360	Protein_149	hypothetical protein	-
HO392_RS23410	143277..143483	+	207	WP_000547965.1	hypothetical protein	-
HO392_RS22865	143508..143795	+	288	WP_000107542.1	hypothetical protein	-
HO392_RS22870	143914..144735	+	822	WP_001234417.1	DUF945 domain-containing protein	-
HO392_RS22875	145031..145621	-	591	WP_170919186.1	transglycosylase SLT domain-containing protein	-
HO392_RS22880	145954..146337	+	384	WP_001151527.1	relaxosome protein TraM	-
HO392_RS22885	146524..147213	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B)	yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC	1..242042	242042

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T157208 WP_001302184.1 NZ_CP053608:142452-142610 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T157208 NZ_CP069078:c1224874-1224563 [Mycobacterium tuberculosis]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACATCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT157208 NZ_CP053608:142189-142387 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin