157205

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 7876..8570 Replicon plasmid F'Iq
Accession NZ_CP053608
Organism Escherichia coli strain NEB5-alpha_F'Iq

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag HO392_RS22150 Protein ID WP_001263489.1
Coordinates 8172..8570 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag HO392_RS22145 Protein ID WP_000554758.1
Coordinates 7876..8169 (+) Length 98 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO392_RS22125 3508..4005 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
HO392_RS22130 4229..5941 - 1713 Protein_6 flagellar biosynthesis protein FlhA -
HO392_RS22135 5913..6698 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
HO392_RS22140 6769..7824 + 1056 WP_001226164.1 DNA polymerase IV -
HO392_RS22145 7876..8169 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
HO392_RS22150 8172..8570 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
HO392_RS22155 8580..9032 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
HO392_RS22160 9350..9556 + 207 Protein_12 RtcB family protein -
HO392_RS22165 9552..10073 + 522 Protein_13 peptide chain release factor H -
HO392_RS22170 10130..11587 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
HO392_RS22175 11848..12306 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 12902..12982 + 81 NuclAT_1 - -
- 12902..12982 + 81 NuclAT_1 - -
- 12902..12982 + 81 NuclAT_1 - -
- 12902..12982 + 81 NuclAT_1 - -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(B) yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC 1..242042 242042


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-130)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T157205 WP_001263489.1 NZ_CP053608:8172-8570 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T157205 NZ_CP069078:835889-836317 [Mycobacterium tuberculosis]
ATGTTCCTTCTCGACGCCAACGTGCTGCTGGCTGCACACCGCGGTGACCACCCGAATCACCGAACCGTCCGCCCCTGGTT
CGATCGACTGCTCGCGGCTGACGACCCCTTCACAGTGCCGAACCTGGTATGGGCGTCGTTCCTCCGGCTGGCAACGAATC
GACGCATCTTCGAGATTCCGTCACCGCGAGCAGAGGCATTCGCATTCGTCGAAGCCGTCACCGCCCAGCCCCATCACCTT
CCGACGAACCCCGGTCCCAGACACCTGGTGCTGCTGCGAAAACTCTGCGACGAGGCCGACGCATCGGGCGACTTGATACC
TGACGCGGTACTCGCGGCCATAGCAGTGGGGCATCACTGCGCCGTGGTGAGCCTGGACAGGGATTTCGCCCGGTTTGCCT
CGGTGCGCCACATTCGCCCGCCGCTCTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT157205 WP_000554758.1 NZ_CP053608:7876-8169 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT157205 NZ_CP069078:835608-835865 [Mycobacterium tuberculosis]
ATGCGCACCACGGTGTCAATCTCCGATGAAATACTCGCTGCCGCCAAACGCCGGGCCCGCGAGCGTGGTCAATCGCTGGG
CGCTGTGATCGAGGACGCCCTTCGGCGGGAGTTCGCCGCCGCCCACGTCGGCGGCGCCCGCCCGACCGTCCCGGTTTTCG
ACGGCGGCACCGGTCCGCGGCGAGGCATCGACCTGACCTCGAATAGGGTGTTGTCCGAAGTGCTCGACGAGGGCCTGGAA
CTGAACTCCCGGAAGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A090J8B1


Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References