Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 253343..254037 Replicon chromosome
Accession NZ_CP053607
Organism Escherichia coli strain NEB5-alpha_F'Iq

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag HO392_RS01180 Protein ID WP_001263489.1
Coordinates 253639..254037 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag HO392_RS01175 Protein ID WP_000554758.1
Coordinates 253343..253636 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO392_RS01155 248975..249472 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
HO392_RS01160 249696..251408 - 1713 Protein_226 flagellar biosynthesis protein FlhA -
HO392_RS01165 251380..252165 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
HO392_RS01170 252236..253291 + 1056 WP_001226164.1 DNA polymerase IV -
HO392_RS01175 253343..253636 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
HO392_RS01180 253639..254037 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
HO392_RS01185 254047..254499 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
HO392_RS01190 254817..255023 + 207 Protein_232 RtcB family protein -
HO392_RS01195 255019..255540 + 522 Protein_233 peptide chain release factor H -
HO392_RS01200 255597..257054 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
HO392_RS01205 257315..257773 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 258369..258449 + 81 NuclAT_12 - -
- 258369..258449 + 81 NuclAT_12 - -
- 258369..258449 + 81 NuclAT_12 - -
- 258369..258449 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T157162 WP_001263489.1 NZ_CP053607:253639-254037 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T157162 NZ_CP069077:c2215846-2215550 [Mycobacterium tuberculosis]
GTGAGTAGCCGATACCTTCTCTCGCCTGCCGCGCAGGCACATCTGGAAGAGATCTGGGACTGCACCTATGACCGTTGGGG
TGTCGATCAGGCCGAGCAGTACCTGCGCGAACTTCAACACGCTATCGACCGTGCTGCGGCAAACCCGCGGATCGGACGAG
CGTGCGACGAGATTCGCCCCGGCTATCGCAAGCTCTCGGCCGGGTCACACACGTTGTTCTATCGGGTGACTGGCGAAGGC
ACCATCGACGTCGTGCGAGTCCTGCACCAACGGATGGACGTCGACCGGAACCTCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT157162 WP_000554758.1 NZ_CP053607:253343-253636 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT157162 NZ_CP069077:c2216094-2215843 [Mycobacterium tuberculosis]
GTGGGTAAGAACACGTCCTTCGTCCTCGACGAGCACTACAGCGCCTTCATCGACGGCGAGATCGCCGCGGGCCGCTACCG
GTCGGCCAGTGAAGTCATCCGCTCCGCGTTGCGACTGCTCGAGGACCGTGAAACCCAGCTGCGCGCGCTTCGTGAGGCTC
TCGAGGCCGGCGAACGCAGCGGCAGCTCGACACCGTTCGACTTCGACGGATTCCTCGGTCGTAAGCGGGCTGACGCCTCG
CGTGGCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References