Detailed information of TA system TA157156

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 133033..133454
• Replicon: plasmid F'
• Accession: NZ_CP053606
• Organism: Escherichia coli strain NEB_Turbo

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: HO393_RS22470
• Protein ID: WP_001302184.1
• Coordinates: 133296..133454 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	133033..133231 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HO393_RS22440	128276..128674	+	399	WP_010892538.1	hypothetical protein	-
HO393_RS22445	128963..129502	+	540	WP_000290801.1	single-stranded DNA-binding protein	-
HO393_RS22450	129559..129792	+	234	WP_000005990.1	DUF905 family protein	-
HO393_RS22455	129858..131816	+	1959	WP_000117173.1	ParB/RepB/Spo0J family partition protein	-
HO393_RS22460	131871..132305	+	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
HO393_RS22465	132302..133021	+	720	WP_000116348.1	plasmid SOS inhibition protein A	-
-	133033..133231	+	199	NuclAT_0	-	Antitoxin
-	133033..133231	+	199	NuclAT_0	-	Antitoxin
-	133033..133231	+	199	NuclAT_0	-	Antitoxin
-	133033..133231	+	199	NuclAT_0	-	Antitoxin
HO393_RS22470	133296..133454	+	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
HO393_RS22475	133692..134051	-	360	Protein_139	hypothetical protein	-
HO393_RS22480	134052..134288	-	237	Protein_140	hypothetical protein	-
HO393_RS22485	134352..134639	+	288	WP_000107542.1	hypothetical protein	-
HO393_RS22490	134758..135579	+	822	WP_001234417.1	DUF945 domain-containing protein	-
HO393_RS22495	135875..136465	-	591	WP_170919186.1	transglycosylase SLT domain-containing protein	-
HO393_RS22500	136798..137181	+	384	WP_001151527.1	relaxosome protein TraM	-
HO393_RS22505	137368..138057	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC	1..231547	231547

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T157156 WP_001302184.1 NZ_CP053606:133296-133454 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T157156 NZ_CP069077:1691676-1691993 [Mycobacterium tuberculosis]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin

Nucleotide

• Length: 199 bp

>AT157156 NZ_CP053606:133033-133231 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin