Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 7877..8571 Replicon plasmid F'
Accession NZ_CP053606
Organism Escherichia coli strain NEB_Turbo

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag HO393_RS21825 Protein ID WP_001263489.1
Coordinates 8173..8571 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag HO393_RS21820 Protein ID WP_000554758.1
Coordinates 7877..8170 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO393_RS21800 3509..4006 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
HO393_RS21805 4230..5969 - 1740 WP_001334802.1 flagellar biosynthesis protein FlhA -
HO393_RS21810 5914..6699 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
HO393_RS21815 6770..7825 + 1056 WP_001226164.1 DNA polymerase IV -
HO393_RS21820 7877..8170 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
HO393_RS21825 8173..8571 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
HO393_RS21830 8581..9033 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
HO393_RS21835 9339..9605 + 267 WP_001295202.1 hypothetical protein -
HO393_RS21840 9517..10074 + 558 WP_001352051.1 peptide chain release factor H -
HO393_RS21845 10131..11588 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
HO393_RS21850 11849..12307 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 12903..12983 + 81 NuclAT_1 - -
- 12903..12983 + 81 NuclAT_1 - -
- 12903..12983 + 81 NuclAT_1 - -
- 12903..12983 + 81 NuclAT_1 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC 1..231547 231547


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T157153 WP_001263489.1 NZ_CP053606:8173-8571 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T157153 NZ_CP069077:c1390673-1390380 [Mycobacterium tuberculosis]
GTGAGCGACGACCATCCCTACCACGTGGCGATCACCGCGACAGCGGCACGCGACCTGCAACGCTTACCCGAAAAGATCGC
CGCCGCATGTGTCGAGTTTGTTTTCGGACCGCTGCTTAACAACCCGCATAGGTTGGGCAAGCCGCTGCGCAATGACCTTG
AAGGCCTCCACTCAGCCCGCCGCGGTGATTACCGCGTCGTCTACGCCATCGACGACGGCCACCACCGAGTCGAGATCATC
CACATCGCTCGTCGCAGTGCCAGCTACCGAATGAACCCGTGCCGGCCACGTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT157153 WP_000554758.1 NZ_CP053606:7877-8170 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT157153 NZ_CP069077:c1390939-1390670 [Mycobacterium tuberculosis]
ATGGCTGTTGTCCCACTGGGCGAAGTCCGCAATCGCCTCTCTGAGTACGTCGCCGAAGTTGAGCTGACACACGAGCGCAT
CACGATAACCCGGCACGGTCATCCGGCGGCGGTATTGATCTCGGCCGATGACCTGGCGTCCATCGAGGAAACGCTGGAGG
TGCTACGCACCCCTGGCGCCAGCGAGGCCATTCGTGAAGGCCTCGCCGATGTTGCCGCAGGGCGCTTCGTGAGCAACGAC
GAGATCCGCAACCGTTACACCGCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References