157141

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1977190..1977692 Replicon chromosome
Accession NZ_CP053605
Organism Escherichia coli strain NEB_Turbo

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HO393_RS09755 Protein ID WP_000767829.1
Coordinates 1977190..1977444 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HO393_RS09760 Protein ID WP_001259255.1
Coordinates 1977441..1977692 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO393_RS09725 1972349..1972576 - 228 WP_000234896.1 sulfurtransferase TusA -
HO393_RS09730 1972590..1973648 - 1059 WP_000492339.1 transport protein YeeE -
HO393_RS09735 1973827..1975185 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
HO393_RS09740 1975175..1975237 - 63 WP_010723108.1 membrane protein YoeI -
HO393_RS09745 1975452..1976237 - 786 WP_171831298.1 LysR family transcriptional regulator -
HO393_RS09750 1976283..1977107 - 825 WP_000754737.1 SDR family oxidoreductase -
HO393_RS09755 1977190..1977444 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HO393_RS09760 1977441..1977692 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HO393_RS09765 1977975..1978025 + 51 WP_001364200.1 his operon leader peptide -
HO393_RS09770 1978119..1978169 + 51 WP_001364200.1 his operon leader peptide -
HO393_RS09775 1978315..1979214 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
HO393_RS09780 1979220..1980524 + 1305 WP_000009594.1 histidinol dehydrogenase -
HO393_RS09785 1980521..1981591 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
HO393_RS09790 1981591..1982658 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T157141 WP_000767829.1 NZ_CP053605:c1977444-1977190 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T157141 NZ_CP069077:720192-720587 [Mycobacterium tuberculosis]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT157141 WP_001259255.1 NZ_CP053605:c1977692-1977441 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT157141 NZ_CP069077:719938-720192 [Mycobacterium tuberculosis]
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References