Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1977190..1977692 Replicon chromosome
Accession NZ_CP053605
Organism Escherichia coli strain NEB_Turbo

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HO393_RS09755 Protein ID WP_000767829.1
Coordinates 1977190..1977444 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HO393_RS09760 Protein ID WP_001259255.1
Coordinates 1977441..1977692 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO393_RS09725 1972349..1972576 - 228 WP_000234896.1 sulfurtransferase TusA -
HO393_RS09730 1972590..1973648 - 1059 WP_000492339.1 transport protein YeeE -
HO393_RS09735 1973827..1975185 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
HO393_RS09740 1975175..1975237 - 63 WP_010723108.1 membrane protein YoeI -
HO393_RS09745 1975452..1976237 - 786 WP_171831298.1 LysR family transcriptional regulator -
HO393_RS09750 1976283..1977107 - 825 WP_000754737.1 SDR family oxidoreductase -
HO393_RS09755 1977190..1977444 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HO393_RS09760 1977441..1977692 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HO393_RS09765 1977975..1978025 + 51 WP_001364200.1 his operon leader peptide -
HO393_RS09770 1978119..1978169 + 51 WP_001364200.1 his operon leader peptide -
HO393_RS09775 1978315..1979214 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
HO393_RS09780 1979220..1980524 + 1305 WP_000009594.1 histidinol dehydrogenase -
HO393_RS09785 1980521..1981591 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
HO393_RS09790 1981591..1982658 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T157141 WP_000767829.1 NZ_CP053605:c1977444-1977190 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T157141 NZ_CP069077:720192-720587 [Mycobacterium tuberculosis]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT157141 WP_001259255.1 NZ_CP053605:c1977692-1977441 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT157141 NZ_CP069077:719938-720192 [Mycobacterium tuberculosis]
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References