Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2180861..2181363 Replicon chromosome
Accession NZ_CP053604
Organism Escherichia coli strain NEB10-beta

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HO394_RS10785 Protein ID WP_000767829.1
Coordinates 2180861..2181115 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HO394_RS10790 Protein ID WP_001259255.1
Coordinates 2181112..2181363 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO394_RS10755 2175876..2176103 - 228 WP_000234896.1 sulfurtransferase TusA -
HO394_RS10760 2176117..2177175 - 1059 WP_000492339.1 transport protein YeeE -
HO394_RS10765 2177354..2178712 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
HO394_RS10770 2178702..2178764 - 63 WP_010723108.1 membrane protein YoeI -
HO394_RS10775 2178979..2179908 - 930 WP_000803351.1 LysR family transcriptional regulator -
HO394_RS10780 2179954..2180778 - 825 WP_000754737.1 SDR family oxidoreductase -
HO394_RS10785 2180861..2181115 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HO394_RS10790 2181112..2181363 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HO394_RS10795 2181646..2181696 + 51 WP_001364200.1 his operon leader peptide -
HO394_RS10800 2181842..2182741 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
HO394_RS10805 2182747..2184051 + 1305 WP_000009594.1 histidinol dehydrogenase -
HO394_RS10810 2184048..2185118 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
HO394_RS10815 2185118..2186185 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T157112 WP_000767829.1 NZ_CP053604:c2181115-2180861 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T157112 NZ_CP069076:c2842124-2841705 [Mycobacterium tuberculosis]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT157112 WP_001259255.1 NZ_CP053604:c2181363-2181112 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT157112 NZ_CP069076:c2842345-2842121 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References