Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 227445..228139 Replicon chromosome
Accession NZ_CP053604
Organism Escherichia coli strain NEB10-beta

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag HO394_RS01070 Protein ID WP_001263489.1
Coordinates 227741..228139 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag HO394_RS01065 Protein ID WP_000554758.1
Coordinates 227445..227738 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO394_RS01045 223077..223574 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
HO394_RS01050 223798..225537 - 1740 WP_001334802.1 flagellar biosynthesis protein FlhA -
HO394_RS01055 225482..226267 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
HO394_RS01060 226338..227393 + 1056 WP_001226164.1 DNA polymerase IV -
HO394_RS01065 227445..227738 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
HO394_RS01070 227741..228139 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
HO394_RS01075 228149..228601 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
HO394_RS01080 228907..229173 + 267 WP_001295202.1 hypothetical protein -
HO394_RS01085 229085..229642 + 558 WP_001352051.1 peptide chain release factor H -
HO394_RS01090 229699..231156 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
HO394_RS01095 231417..231875 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 232471..232551 + 81 NuclAT_12 - -
- 232471..232551 + 81 NuclAT_12 - -
- 232471..232551 + 81 NuclAT_12 - -
- 232471..232551 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T157093 WP_001263489.1 NZ_CP053604:227741-228139 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T157093 NZ_CP069076:1384840-1385271 [Mycobacterium tuberculosis]
GTGATCATCCCTGACATCAATCTGCTGCTCTACGCGGTCATCACCGGATTCCCGCAGCACCGGCGCGCGCATGCGTGGTG
GCAAGACACCGTCAACGGCCACACCCGTATCGGGCTGACGTATCCGGCGTTGTTCGGGTTCCTACGGATCGCCACCAGTG
CCCGCGTGCTCGCCGCGCCACTGCCAACCGCGGATGCGATCGCCTATGTGCGCGAGTGGCTTTCGCAGCCGAACGTGGAC
CTACTCACGGCGGGTCCGCGCCACCTGGACATCGCGTTGGGCCTGCTCGACAAGCTCGGCACAGCCAGCCACCTAACCAC
CGATGTGCAACTGGCCGCCTACGGCATCGAATACGACGCCGAGATCCATTCCAGTGACACCGACTTTGCCCGATTCGCCG
ATCTGAAGTGGACCGACCCGTTGCGCGAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT157093 WP_000554758.1 NZ_CP053604:227445-227738 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT157093 NZ_CP069076:1384583-1384843 [Mycobacterium tuberculosis]
ATGCGCACCACCTTGACGCTCGATGACGACGTCGTCCGGCTGGTCGAAGACGCAGTGCATCGCGAACGCCGCCCGATGAA
GCAGGTCATCAACGATGCGCTGCGCAGAGCGCTGGCGCCGCCGGTGAAACGGCAGGAGCAGTATCGGTTGGAGCCGCATG
AGTCGGCTGTGCGTTCCGGGTTGGATCTGGCCGGCTTCAACAAGTTGGCCGACGAACTGGAGGATGAGGCGCTGCTGGAT
GCCACGCGTCGGGCCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References