Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3515291..3516090 Replicon chromosome
Accession NZ_CP053603
Organism Escherichia coli strain Dam/Dcm

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag HO395_RS16995 Protein ID WP_000347273.1
Coordinates 3515291..3515755 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HO395_RS17000 Protein ID WP_001307405.1
Coordinates 3515755..3516090 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO395_RS16970 3511467..3512024 - 558 Protein_3310 amidohydrolase family protein -
HO395_RS16975 3512020..3512391 - 372 Protein_3311 PTS sugar transporter subunit IIC -
HO395_RS16980 3512402..3512875 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
HO395_RS16985 3512898..3514178 - 1281 WP_001698595.1 tagatose-bisphosphate aldolase subunit KbaZ -
HO395_RS16990 3514427..3515236 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HO395_RS16995 3515291..3515755 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HO395_RS17000 3515755..3516090 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HO395_RS17005 3516239..3517810 - 1572 WP_001273753.1 galactarate dehydratase -
HO395_RS17010 3518185..3519519 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
HO395_RS17015 3519535..3520305 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3504307..3526965 22658


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T157082 WP_000347273.1 NZ_CP053603:c3515755-3515291 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T157082 NZ_CP069076:713127-713522 [Mycobacterium tuberculosis]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT157082 WP_001307405.1 NZ_CP053603:c3516090-3515755 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT157082 NZ_CP069076:712873-713127 [Mycobacterium tuberculosis]
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References