Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3101108..3101835 Replicon chromosome
Accession NZ_CP053602
Organism Escherichia coli BL21(DE3)

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag HO396_RS15040 Protein ID WP_000550189.1
Coordinates 3101521..3101835 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HO396_RS15035 Protein ID WP_000560266.1
Coordinates 3101108..3101524 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO396_RS15025 3096268..3098619 + 2352 WP_000695489.1 alpha-glucosidase -
HO396_RS15030 3099045..3101063 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
HO396_RS15035 3101108..3101524 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
HO396_RS15040 3101521..3101835 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
HO396_RS15045 3102119..3103255 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
HO396_RS15050 3103340..3103843 + 504 WP_001300832.1 M48 family metallopeptidase -
HO396_RS15055 3103920..3104612 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
HO396_RS15060 3104691..3105677 + 987 WP_012775974.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T157046 WP_000550189.1 NZ_CP053602:c3101835-3101521 [Escherichia coli BL21(DE3)]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T157046 NZ_CP069075:c2236801-2236241 [Mycobacterium tuberculosis]
GTGAGCGATGCCCTCGATGAAGGGCTCGTCCAGCGTATCGACGCACGCGGAACAATTGAGTGGTCGGAAACGTGCTACCG
GTATACCGGCGCGCACCGTGACGCCTTGTCCGGTGAGGGCGCGCGCAGATTCGGAGGCAGGTGGAATCCGCCGCTGCTCT
TTCCGGCGATCTATCTTGCTGATTCCGCCCAAGCCTGCATGGTTGAGGTGGAACGGGCGGCGCAAGCGGCTTCAACGACC
GCAGAGAAGATGCTCGAGGCGGCCTACCGACTACACACGATCGACGTCACGGACCTGGCCGTCCTCGATCTGACAACCCC
GCAAGCTCGGGAAGCCGTGGGGCTCGAGAACGACGACATCTATGGCGACGACTGGTCAGGGTGCCAGGCGGTCGGACATG
CGGCCTGGTTCTTGCACATGCAAGGTGTCCTCGTGCCGGCGGCGGGCGGTGTCGGCCTCGTTGTCACCGCGTATGAACAG
CGAACTCGGCCGGGCCAACTACAACTGCGACAAAGCGTCGATCTGACGCCTGCTCTTTACCAAGAACTTCGAGCCACGTA
G

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT157046 WP_000560266.1 NZ_CP053602:c3101524-3101108 [Escherichia coli BL21(DE3)]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT157046 NZ_CP069075:c2237139-2236798 [Mycobacterium tuberculosis]
ATGGGAGTAAATGTGCTCGCCTCGACCGTGTCGGGTGCGATCGAGCGCTTGGGATTGACCTACGAGGAAGTCGGTGACAT
CGTCGATGCCTCGCCGCGTTCCGTGGCGCGATGGACCGCAGGTCAGGTGGTTCCCCAACGCCTCAACAAGCAACGACTTA
TCGAGCTGGCCTATGTCGCCGACGCCCTCGCGGAAGTGCTGCCGCGTGACCAGGCGAACGTGTGGATGTTTTCGCCGAAT
CGGTTACTGGAACACCGCAAGCCTGCCGACCTCGTGCGAGACGGCGAGTACCAACGCGTGTTGGCGCTCATCGACGCGAT
GGCGGAGGGAGTGTTCGTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References