Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mqsRA (relBE)/MqsR-MqsA
Location 3036586..3037279 Replicon chromosome
Accession NZ_CP053602
Organism Escherichia coli BL21(DE3)

Toxin (Protein)


Gene name mqsR Uniprot ID S1EZG2
Locus tag HO396_RS14735 Protein ID WP_000415584.1
Coordinates 3036983..3037279 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name mqsA Uniprot ID S1EBV2
Locus tag HO396_RS14730 Protein ID WP_000650107.1
Coordinates 3036586..3036981 (-) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO396_RS14720 3032450..3034708 - 2259 WP_001281877.1 DNA topoisomerase IV subunit A -
HO396_RS14725 3034846..3036453 - 1608 WP_001295629.1 ABC transporter substrate-binding protein -
HO396_RS14730 3036586..3036981 - 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA Antitoxin
HO396_RS14735 3036983..3037279 - 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR Toxin
HO396_RS14740 3037484..3037966 - 483 WP_000183505.1 transcriptional regulator -
HO396_RS14745 3038019..3038411 - 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
HO396_RS14750 3038563..3039222 + 660 WP_001221493.1 two-component system response regulator QseB -
HO396_RS14755 3039219..3040568 + 1350 WP_000673402.1 two-component system sensor histidine kinase QseC -
HO396_RS14760 3040614..3040946 - 333 WP_000917684.1 DUF2645 family protein -
HO396_RS14765 3041265..3041846 + 582 WP_000072420.1 NADPH:quinone oxidoreductase MdaB -
HO396_RS14770 3041877..3042191 + 315 WP_000958598.1 antibiotic biosynthesis monooxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin MqsR

(5-97)

Antitoxin

Zinc finger, MqsA-type

(3-47)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11231.96 Da        Isoelectric Point: 8.9070

>T157045 WP_000415584.1 NZ_CP053602:c3037279-3036983 [Escherichia coli BL21(DE3)]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Download         Length: 297 bp

>T157045 NZ_CP069075:c2229334-2228915 [Mycobacterium tuberculosis]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 14703.10 Da        Isoelectric Point: 9.2136

>AT157045 WP_000650107.1 NZ_CP053602:c3036981-3036586 [Escherichia coli BL21(DE3)]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Download         Length: 396 bp

>AT157045 NZ_CP069075:c2229603-2229343 [Mycobacterium tuberculosis]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3HI2

Antitoxin

Source ID Structure
PDB 3HI2
PDB 3GA8
PDB 3O9X
PDB 2KZ8
PDB 3FMY
PDB 3GN5
AlphaFold DB A0A7U9LU65

References