Detailed information of TA system TA157024

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 257869..258563
• Replicon: chromosome
• Accession: NZ_CP053602
• Organism: Escherichia coli BL21(DE3)

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: HO396_RS01180
• Protein ID: WP_001263493.1
• Coordinates: 258165..258563 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: HO396_RS01175
• Protein ID: WP_000554757.1
• Coordinates: 257869..258162 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HO396_RS01155	253509..254006	+	498	WP_000006260.1	REP-associated tyrosine transposase RayT	-
HO396_RS01160	254222..255961	-	1740	WP_001301256.1	flagellar biosynthesis protein FlhA	-
HO396_RS01165	255906..256691	+	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
HO396_RS01170	256762..257817	+	1056	WP_001226164.1	DNA polymerase IV	-
HO396_RS01175	257869..258162	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HO396_RS01180	258165..258563	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HO396_RS01185	258573..259025	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
HO396_RS01190	259259..259525	+	267	WP_001321003.1	hypothetical protein	-
HO396_RS01195	259437..259994	+	558	WP_001353768.1	peptide chain release factor H	-
HO396_RS01200	260051..261508	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
HO396_RS01205	261769..262227	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
HO396_RS01210	262319..263563	+	1245	WP_000189541.1	esterase FrsA	-
-	262823..262903	+	81	NuclAT_13	-	-
-	262823..262903	+	81	NuclAT_13	-	-
-	262823..262903	+	81	NuclAT_13	-	-
-	262823..262903	+	81	NuclAT_13	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T157024 WP_001263493.1 NZ_CP053602:258165-258563 [Escherichia coli BL21(DE3)]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T157024 NZ_CP069075:722837-723232 [Mycobacterium tuberculosis]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT157024 WP_000554757.1 NZ_CP053602:257869-258162 [Escherichia coli BL21(DE3)]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT157024 NZ_CP069075:722583-722837 [Mycobacterium tuberculosis]
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6