Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 257868..258562 Replicon chromosome
Accession NZ_CP053601
Organism Escherichia coli BL21

Toxin (Protein)


Gene name yafO Uniprot ID S1EXB8
Locus tag HO397_RS01180 Protein ID WP_001263493.1
Coordinates 258164..258562 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag HO397_RS01175 Protein ID WP_000554757.1
Coordinates 257868..258161 (+) Length 98 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO397_RS01155 253508..254005 + 498 WP_000006260.1 REP-associated tyrosine transposase RayT -
HO397_RS01160 254221..255960 - 1740 WP_001301256.1 flagellar biosynthesis protein FlhA -
HO397_RS01165 255905..256690 + 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -
HO397_RS01170 256761..257816 + 1056 WP_001226164.1 DNA polymerase IV -
HO397_RS01175 257868..258161 + 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
HO397_RS01180 258164..258562 + 399 WP_001263493.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
HO397_RS01185 258572..259024 + 453 WP_001059847.1 GNAT family N-acetyltransferase -
HO397_RS01190 259258..259524 + 267 WP_001321003.1 hypothetical protein -
HO397_RS01195 259436..259993 + 558 WP_001353768.1 peptide chain release factor H -
HO397_RS01200 260050..261507 - 1458 WP_001293003.1 cytosol nonspecific dipeptidase -
HO397_RS01205 261768..262226 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
HO397_RS01210 262318..263562 + 1245 WP_000189541.1 esterase FrsA -
- 262822..262902 + 81 NuclAT_13 - -
- 262822..262902 + 81 NuclAT_13 - -
- 262822..262902 + 81 NuclAT_13 - -
- 262822..262902 + 81 NuclAT_13 - -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-130)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 133 a.a.        Molecular weight: 15472.83 Da        Isoelectric Point: 8.0949

>T156998 WP_001263493.1 NZ_CP053601:258164-258562 [Escherichia coli BL21]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T156998 NZ_CP069074:c2896051-2895656 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT156998 WP_000554757.1 NZ_CP053601:257868-258161 [Escherichia coli BL21]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT156998 NZ_CP069074:c2896293-2896048 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB S1EXB8


Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References