Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3101545..3102272 Replicon chromosome
Accession NZ_CP053600
Organism Escherichia coli strain Nico21(DE3)

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag HO398_RS15045 Protein ID WP_000550189.1
Coordinates 3101958..3102272 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HO398_RS15040 Protein ID WP_000560266.1
Coordinates 3101545..3101961 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO398_RS15030 3096705..3099056 + 2352 WP_000695489.1 alpha-glucosidase -
HO398_RS15035 3099482..3101500 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
HO398_RS15040 3101545..3101961 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
HO398_RS15045 3101958..3102272 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
HO398_RS15050 3102556..3103692 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
HO398_RS15055 3103777..3104280 + 504 WP_001300832.1 M48 family metallopeptidase -
HO398_RS15060 3104357..3105049 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
HO398_RS15065 3105128..3106114 + 987 WP_012775974.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T156994 WP_000550189.1 NZ_CP053600:c3102272-3101958 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T156994 NZ_CP069074:2834215-2834640 [Mycobacterium tuberculosis]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGTGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACGGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT156994 WP_000560266.1 NZ_CP053600:c3101961-3101545 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT156994 NZ_CP069074:2833988-2834209 [Mycobacterium tuberculosis]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References