Detailed information of TA system TA156993

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 3037023..3037716
• Replicon: chromosome
• Accession: NZ_CP053600
• Organism: Escherichia coli strain Nico21(DE3)

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: HO398_RS14740
• Protein ID: WP_000415584.1
• Coordinates: 3037420..3037716 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: HO398_RS14735
• Protein ID: WP_000650107.1
• Coordinates: 3037023..3037418 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HO398_RS14725	3032887..3035145	-	2259	WP_001281877.1	DNA topoisomerase IV subunit A	-
HO398_RS14730	3035283..3036890	-	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
HO398_RS14735	3037023..3037418	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
HO398_RS14740	3037420..3037716	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
HO398_RS14745	3037921..3038403	-	483	WP_000183505.1	transcriptional regulator	-
HO398_RS14750	3038456..3038848	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
HO398_RS14755	3039000..3039659	+	660	WP_001221493.1	two-component system response regulator QseB	-
HO398_RS14760	3039656..3041005	+	1350	WP_000673402.1	two-component system sensor histidine kinase QseC	-
HO398_RS14765	3041051..3041383	-	333	WP_000917684.1	DUF2645 family protein	-
HO398_RS14770	3041702..3042283	+	582	WP_000072420.1	NADPH:quinone oxidoreductase MdaB	-
HO398_RS14775	3042314..3042628	+	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T156993 WP_000415584.1 NZ_CP053600:c3037716-3037420 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T156993 NZ_CP069074:c2431091-2430774 [Mycobacterium tuberculosis]
ATGACGCGCAGGCTGCGCGTCCATAACGGGGTTGAAGACGACCTATTCGAGGCGTTTTCCTACTACGCGGACGCGGCGCC
AGATCAGATCGATCGGCTTTACAACTTGTTTGTCGATGCCGTGACGAAGCGGATTCCGCAGGCTCCGAACGCGTTTGCGC
CGTTATTCAAGCACTATCGACACATCTACCTCCGGCCCTTCAGGTACTACGTTGCCTATCGGACGACCGACGAGGCTATC
GACATACTGGCTGTTCGGCACGGAATGGAGAACCCGAACGCGGTCGAGGCTGAGATCTCTGGCCGCACCTTCGAGTGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT156993 WP_000650107.1 NZ_CP053600:c3037418-3037023 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT156993 NZ_CP069074:c2431303-2431088 [Mycobacterium tuberculosis]
GTGGTGGTCAACCGGGCATTGCTGGCGAGCGTCGACGCACTGTCGCGTGATGAGCAGATTGAGCTCGTCGAGCACATCAA
CGGAAACCTAGCCGAGGGCATGCATATCAGCGAGGCCAACCAGGCGCTCATCGAAGCGCGGGCCAATGACACCGACGATG
CTCATTGGTCCACCATTGATGACTTCGACAAGCGGATCCGCGCCCGGCTCGGATGA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65