Detailed information of TA system TA156972

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 258081..258775
• Replicon: chromosome
• Accession: NZ_CP053600
• Organism: Escherichia coli strain Nico21(DE3)

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: HO398_RS01185
• Protein ID: WP_001263493.1
• Coordinates: 258377..258775 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: HO398_RS01180
• Protein ID: WP_000554757.1
• Coordinates: 258081..258374 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HO398_RS01160	253721..254218	+	498	WP_000006260.1	REP-associated tyrosine transposase RayT	-
HO398_RS01165	254434..256146	-	1713	Protein_226	flagellar biosynthesis protein FlhA	-
HO398_RS01170	256118..256903	+	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
HO398_RS01175	256974..258029	+	1056	WP_001226164.1	DNA polymerase IV	-
HO398_RS01180	258081..258374	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HO398_RS01185	258377..258775	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HO398_RS01190	258785..259237	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
HO398_RS01195	259483..259686	+	204	Protein_232	RNA ligase RtcB family protein	-
HO398_RS01200	259685..260206	+	522	Protein_233	peptide chain release factor H	-
HO398_RS01205	260263..261720	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
HO398_RS01210	261981..262439	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
HO398_RS01215	262531..263775	+	1245	WP_000189541.1	esterase FrsA	-
-	263035..263115	+	81	NuclAT_13	-	-
-	263035..263115	+	81	NuclAT_13	-	-
-	263035..263115	+	81	NuclAT_13	-	-
-	263035..263115	+	81	NuclAT_13	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T156972 WP_001263493.1 NZ_CP053600:258377-258775 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T156972 NZ_CP069074:1020056-1020490 [Mycobacterium tuberculosis]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT156972 WP_000554757.1 NZ_CP053600:258081-258374 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT156972 NZ_CP069074:1019871-1020050 [Mycobacterium tuberculosis]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCATGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6