Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4154244..4154785 Replicon chromosome
Accession NZ_CP053599
Organism Escherichia coli strain NEBExpress

Toxin (Protein)


Gene name yafQ Uniprot ID A0A140NAP5
Locus tag HO399_RS20050 Protein ID WP_000615981.1
Coordinates 4154507..4154785 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag HO399_RS20045 Protein ID WP_000729704.1
Coordinates 4154244..4154504 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO399_RS20025 4149927..4150712 - 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -
HO399_RS20030 4150657..4152396 + 1740 WP_001301256.1 flagellar biosynthesis protein FlhA -
HO399_RS20035 4152612..4153109 - 498 WP_000006260.1 REP-associated tyrosine transposase RayT -
HO399_RS20040 4153285..4154058 - 774 WP_001353765.1 C40 family peptidase -
HO399_RS20045 4154244..4154504 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HO399_RS20050 4154507..4154785 + 279 WP_000615981.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HO399_RS20055 4154941..4155681 + 741 WP_001225679.1 murein L,D-transpeptidase -
HO399_RS20060 4155652..4156419 - 768 WP_000333379.1 class II glutamine amidotransferase -
HO399_RS20065 4156625..4157203 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10860.65 Da        Isoelectric Point: 10.0702

>T156969 WP_000615981.1 NZ_CP053599:4154507-4154785 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQSSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T156969 NZ_CP069074:c760564-760127 [Mycobacterium tuberculosis]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT156969 WP_000729704.1 NZ_CP053599:4154244-4154504 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT156969 NZ_CP069074:c760017-759772 [Mycobacterium tuberculosis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MMG
PDB 4MMJ
PDB 4ML2
PDB 4ML0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References