Detailed information of TA system TA156968

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4148055..4148749
• Replicon: chromosome
• Accession: NZ_CP053599
• Organism: Escherichia coli strain NEBExpress

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: HO399_RS20010
• Protein ID: WP_001263493.1
• Coordinates: 4148055..4148453 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: HO399_RS20015
• Protein ID: WP_000554757.1
• Coordinates: 4148456..4148749 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HO399_RS19980	4143055..4144299	-	1245	WP_000189541.1	esterase FrsA	-
-	4143715..4143795	-	81	NuclAT_13	-	-
-	4143715..4143795	-	81	NuclAT_13	-	-
-	4143715..4143795	-	81	NuclAT_13	-	-
-	4143715..4143795	-	81	NuclAT_13	-	-
HO399_RS19985	4144391..4144849	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
HO399_RS19990	4145110..4146567	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
HO399_RS19995	4146624..4147145	-	522	Protein_3890	peptide chain release factor H	-
HO399_RS20000	4147144..4147347	-	204	Protein_3891	RNA ligase RtcB family protein	-
HO399_RS20005	4147593..4148045	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
HO399_RS20010	4148055..4148453	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HO399_RS20015	4148456..4148749	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HO399_RS20020	4148801..4149856	-	1056	WP_001226164.1	DNA polymerase IV	-
HO399_RS20025	4149927..4150712	-	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
HO399_RS20030	4150657..4152396	+	1740	WP_001301256.1	flagellar biosynthesis protein FlhA	-
HO399_RS20035	4152612..4153109	-	498	WP_000006260.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T156968 WP_001263493.1 NZ_CP053599:c4148453-4148055 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T156968 NZ_CP069074:c759785-759477 [Mycobacterium tuberculosis]
ATGCGGCGCGGTGAATTGTGGTTTGCCGCCACACCTGGTGGTGACAGACCAGTACTTGTCCTTACCAGAGATCCGGTGGC
AGACCGCATCGGCGCGGTCGTTGTGGTGGCCCTAACCCGCACCCGCCGAGGCCTGGTGTCGGAATTGGAGCTCACGGCCG
TCGAAAACCGTGTTCCGAGCGACTGCGTCGTCAACTTCGACAACATTCATACGTTGCCACGCACCGCATTCCGACGCCGC
ATCACCCGGCTGTCCCCGGCCCGCCTGCACGAAGCCTGTCAAACACTCCGGGCGAGCACGGGGTGTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT156968 WP_000554757.1 NZ_CP053599:c4148749-4148456 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT156968 NZ_CP069074:c760017-759772 [Mycobacterium tuberculosis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6