Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4156519..4157060 Replicon chromosome
Accession NZ_CP053594
Organism Escherichia coli strain T7Express_Crystal

Toxin (Protein)


Gene name yafQ Uniprot ID A0A140NAP5
Locus tag HO402_RS20085 Protein ID WP_000615981.1
Coordinates 4156782..4157060 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag HO402_RS20080 Protein ID WP_000729704.1
Coordinates 4156519..4156779 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO402_RS20060 4152202..4152987 - 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -
HO402_RS20065 4152932..4154671 + 1740 WP_001301256.1 flagellar biosynthesis protein FlhA -
HO402_RS20070 4154887..4155384 - 498 WP_000006260.1 REP-associated tyrosine transposase RayT -
HO402_RS20075 4155560..4156333 - 774 WP_001353765.1 C40 family peptidase -
HO402_RS20080 4156519..4156779 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HO402_RS20085 4156782..4157060 + 279 WP_000615981.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HO402_RS20090 4157216..4157956 + 741 WP_001225679.1 murein L,D-transpeptidase -
HO402_RS20095 4157927..4158694 - 768 WP_000333379.1 class II glutamine amidotransferase -
HO402_RS20100 4158900..4159478 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10860.65 Da        Isoelectric Point: 10.0702

>T156929 WP_000615981.1 NZ_CP053594:4156782-4157060 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQSSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T156929 NZ_CP069073:c2237279-2236719 [Mycobacterium tuberculosis]
GTGAGCGATGCCCTCGATGAAGGGCTCGTCCAGCGTATCGACGCACGCGGAACAATTGAGTGGTCGGAAACGTGCTACCG
GTATACCGGCGCGCACCGTGACGCCTTGTCCGGTGAGGGCGCGCGCAGATTCGGAGGCAGGTGGAATCCGCCGCTGCTCT
TTCCGGCGATCTATCTTGCTGATTCCGCCCAAGCCTGCATGGTTGAGGTGGAACGGGCGGCGCAAGCGGCTTCAACGACC
GCAGAGAAGATGCTCGAGGCGGCCTACCGACTACACACGATCGACGTCACGGACCTGGCCGTCCTCGATCTGACAACCCC
GCAAGCTCGGGAAGCCGTGGGGCTCGAGAACGACGACATCTATGGCGACGACTGGTCAGGGTGCCAGGCGGTCGGACATG
CGGCCTGGTTCTTGCACATGCAAGGTGTCCTCGTGCCGGCGGCGGGCGGTGTCGGCCTCGTTGTCACCGCGTATGAACAG
CGAACTCGGCCGGGCCAACTACAACTGCGACAAAGCGTCGATCTGACGCCTGCTCTTTACCAAGAACTTCGAGCCACGTA
G

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT156929 WP_000729704.1 NZ_CP053594:4156519-4156779 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT156929 NZ_CP069073:c2237617-2237276 [Mycobacterium tuberculosis]
ATGGGAGTAAATGTGCTCGCCTCGACCGTGTCGGGTGCGATCGAGCGCTTGGGATTGACCTACGAGGAAGTCGGTGACAT
CGTCGATGCCTCGCCGCGTTCCGTGGCGCGATGGACCGCAGGTCAGGTGGTTCCCCAACGCCTCAACAAGCAACGACTTA
TCGAGCTGGCCTATGTCGCCGACGCCCTCGCGGAAGTGCTGCCGCGTGACCAGGCGAACGTGTGGATGTTTTCGCCGAAT
CGGTTACTGGAACACCGCAAGCCTGCCGACCTCGTGCGAGACGGCGAGTACCAACGCGTGTTGGCGCTCATCGACGCGAT
GGCGGAGGGAGTGTTCGTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MMG
PDB 4MMJ
PDB 4ML2
PDB 4ML0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References