Detailed information of TA system TA156928

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4150330..4151024
• Replicon: chromosome
• Accession: NZ_CP053594
• Organism: Escherichia coli strain T7Express_Crystal

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: HO402_RS20045
• Protein ID: WP_001263493.1
• Coordinates: 4150330..4150728 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: HO402_RS20050
• Protein ID: WP_000554757.1
• Coordinates: 4150731..4151024 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HO402_RS20015	4145330..4146574	-	1245	WP_000189541.1	esterase FrsA	-
-	4145990..4146070	-	81	NuclAT_13	-	-
-	4145990..4146070	-	81	NuclAT_13	-	-
-	4145990..4146070	-	81	NuclAT_13	-	-
-	4145990..4146070	-	81	NuclAT_13	-	-
HO402_RS20020	4146666..4147124	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
HO402_RS20025	4147385..4148842	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
HO402_RS20030	4148899..4149456	-	558	WP_001353768.1	peptide chain release factor H	-
HO402_RS20035	4149368..4149634	-	267	WP_001321003.1	hypothetical protein	-
HO402_RS20040	4149868..4150320	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
HO402_RS20045	4150330..4150728	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HO402_RS20050	4150731..4151024	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HO402_RS20055	4151076..4152131	-	1056	WP_001226164.1	DNA polymerase IV	-
HO402_RS20060	4152202..4152987	-	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
HO402_RS20065	4152932..4154671	+	1740	WP_001301256.1	flagellar biosynthesis protein FlhA	-
HO402_RS20070	4154887..4155384	-	498	WP_000006260.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T156928 WP_001263493.1 NZ_CP053594:c4150728-4150330 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T156928 NZ_CP069073:c2229812-2229393 [Mycobacterium tuberculosis]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT156928 WP_000554757.1 NZ_CP053594:c4151024-4150731 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT156928 NZ_CP069073:c2230081-2229821 [Mycobacterium tuberculosis]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6