Detailed information of TA system TA156908

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4148025..4148719
• Replicon: chromosome
• Accession: NZ_CP053592
• Organism: Escherichia coli strain NEBExpress_Iq

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: HO403_RS20035
• Protein ID: WP_001263493.1
• Coordinates: 4148025..4148423 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: HO403_RS20040
• Protein ID: WP_000554757.1
• Coordinates: 4148426..4148719 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HO403_RS20005	4143025..4144269	-	1245	WP_000189541.1	esterase FrsA	-
-	4143685..4143765	-	81	NuclAT_13	-	-
-	4143685..4143765	-	81	NuclAT_13	-	-
-	4143685..4143765	-	81	NuclAT_13	-	-
-	4143685..4143765	-	81	NuclAT_13	-	-
HO403_RS20010	4144361..4144819	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
HO403_RS20015	4145080..4146537	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
HO403_RS20020	4146594..4147151	-	558	WP_001353768.1	peptide chain release factor H	-
HO403_RS20025	4147063..4147329	-	267	WP_001321003.1	hypothetical protein	-
HO403_RS20030	4147563..4148015	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
HO403_RS20035	4148025..4148423	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HO403_RS20040	4148426..4148719	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HO403_RS20045	4148771..4149826	-	1056	WP_001226164.1	DNA polymerase IV	-
HO403_RS20050	4149897..4150682	-	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
HO403_RS20055	4150627..4152366	+	1740	WP_001301256.1	flagellar biosynthesis protein FlhA	-
HO403_RS20060	4152582..4153079	-	498	WP_000006260.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T156908 WP_001263493.1 NZ_CP053592:c4148423-4148025 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T156908 NZ_CP069073:723262-723669 [Mycobacterium tuberculosis]
GTGAGCACGACGCCGGCCGCCGGAGTGCTCGACACGTCGGTGTTCATCGCGACCGAAAGCGGCCGGCAACTCGACGAGGC
GCTGATCCCCGACCGGGTCGCCACCACCGTCGTCACCCTCGCCGAACTGCGCGTCGGCGTGCTGGCCGCGGCGACGACCG
ACATCCGGGCTCAACGCCTGGCGACCCTGGAATCCGTTGCCGATATGGAAACGTTGCCCGTCGACGACGATGCCGCCCGA
ATGTGGGCCCGATTGCGGATCCATCTTGCCGAGTCCGGTCGCCGGGTGCGGATCAACGACCTGTGGATCGCGGCCGTCGC
GGCATCGCGAGCGCTGCCGGTCATCACCCAGGACGACGACTTCGCCGCCCTCGACGGTGCGGCCAGTGTGGAGATCATTC
GGGTCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT156908 WP_000554757.1 NZ_CP053592:c4148719-4148426 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT156908 NZ_CP069073:723005-723265 [Mycobacterium tuberculosis]
ATGTCTGAGGTGGCCTCGCGTGAGCTGCGTAACGATACGGCCGGCGTGCTGCGCCGCGTGCGGGCAGGGGAGGACGTCAC
CATCACCGTCAGCGGCCGTCCGGTCGCGGTGCTTACCCCGGTTCGTCCGCGGCGCCGGCGTTGGCTGAGCAAAACGGAGT
TCCTGTCGCGGTTGCGCGGCGCTCAAGCCGATCCCGGGCTCCGTAACGACCTCGCGGTCCTTGCCGGCGACACGACCGAG
GATCTCGGGCCGATCCGGTGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6