Detailed information of TA system TA156880

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4835538..4836298
• Replicon: chromosome
• Accession: NZ_CP053573
• Organism: Citrobacter sp. TSA-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7L6U900
• Locus tag: HF677_RS22885
• Protein ID: WP_019077936.1
• Coordinates: 4835538..4835849 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HF677_RS22890
• Protein ID: WP_080859878.1
• Coordinates: 4835846..4836298 (+)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF677_RS22855	4831217..4832119	+	903	WP_003028691.1	formate dehydrogenase O subunit beta	-
HF677_RS22860	4832116..4832751	+	636	WP_003028686.1	formate dehydrogenase cytochrome b556 subunit	-
HF677_RS22865	4832748..4833677	+	930	WP_003028685.1	formate dehydrogenase accessory protein FdhE	-
HF677_RS22870	4833714..4834077	-	364	Protein_4472	type II toxin-antitoxin system VapC family toxin	-
HF677_RS22875	4834077..4834319	-	243	WP_016155181.1	ribbon-helix-helix domain-containing protein	-
HF677_RS22880	4834524..4835453	+	930	WP_080859881.1	alpha/beta hydrolase	-
HF677_RS22885	4835538..4835849	+	312	WP_019077936.1	type II toxin-antitoxin system HigB family toxin	Toxin
HF677_RS22890	4835846..4836298	+	453	WP_080859878.1	helix-turn-helix domain-containing protein	Antitoxin
HF677_RS22895	4836316..4837257	-	942	WP_003825286.1	fatty acid biosynthesis protein FabY	-
HF677_RS22900	4837302..4837739	-	438	WP_016157874.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HF677_RS22905	4837736..4838608	-	873	WP_019077938.1	virulence factor BrkB family protein	-
HF677_RS22910	4838602..4839201	-	600	WP_016157873.1	glucose-1-phosphatase	-
HF677_RS22915	4839306..4840109	-	804	WP_016155173.1	DeoR/GlpR family DNA-binding transcription regulator	-
HF677_RS22920	4840143..4841039	-	897	WP_080859876.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (95-148)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12502.67 Da
• Isoelectric Point: 9.9988

>T156880 WP_019077936.1 NZ_CP053573:4835538-4835849 [Citrobacter sp. TSA-1]
MHVISRKPFNEAILRFPNHMAALVDLLNILEKKMFHTPEEMKQYIPSLDNFKYRNKWWVINVSGNCLRLIAYIDFKLQKV
FVKHIVHHAEYDRLTTYYRGHKE

Nucleotide

• Length: 312 bp

>T156880 NZ_CP053573:4835538-4835849 [Citrobacter sp. TSA-1]
ATGCATGTTATCAGCCGAAAACCTTTCAACGAGGCAATACTCCGTTTTCCAAACCACATGGCAGCACTCGTTGATTTGCT
GAATATTTTAGAGAAAAAAATGTTCCACACTCCTGAAGAGATGAAGCAGTACATACCTTCACTGGATAATTTTAAATACA
GAAATAAATGGTGGGTGATTAATGTTTCAGGTAACTGCTTGAGACTTATTGCCTATATTGATTTCAAACTGCAAAAGGTA
TTTGTGAAGCATATCGTTCACCATGCCGAGTATGACAGACTAACCACATACTATCGGGGTCACAAAGAATGA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16967.38 Da
• Isoelectric Point: 6.2277

>AT156880 WP_080859878.1 NZ_CP053573:4835846-4836298 [Citrobacter sp. TSA-1]
MRTHESHQIDTASVKLMIDTFTDAVKKIPLLGKEQNEAEYRKALALVEFLVDRNDLENPLFELLSARIRDYEKHAPEFSA
LNQQLEHTPHGVALLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVSHIKMLAERFNLPADAFIE

Nucleotide

• Length: 453 bp

>AT156880 NZ_CP053573:4835846-4836298 [Citrobacter sp. TSA-1]
ATGAGAACGCATGAAAGCCATCAGATAGACACAGCCAGTGTAAAATTGATGATCGATACCTTTACCGATGCAGTGAAAAA
AATCCCCCTGTTAGGCAAAGAACAAAATGAGGCAGAGTACCGAAAAGCACTCGCTTTGGTGGAGTTTCTGGTTGATCGTA
ACGACCTGGAAAACCCGTTGTTTGAGCTGCTTTCAGCTCGGATCCGCGACTACGAAAAACATGCGCCTGAGTTCAGTGCC
CTCAACCAACAACTGGAACATACTCCTCATGGCGTTGCGTTACTGCGTACGCTCATGGATCAATATGGTCTCAAAGCAGC
AGATCTTGCCAACGAACTTGGTTCAAAATCCAATGTCAGCAACATCTTGAATGGTCGCAGAGCACTGACTGTAAGTCATA
TTAAAATGCTCGCAGAACGCTTTAACCTTCCGGCAGACGCATTCATCGAGTAA

Structures

Toxin

• AlphaFold DB: A0A7L6U900