Detailed information of TA system TA156866

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3298286..3298946
• Replicon: chromosome
• Accession: NZ_CP053572
• Organism: Serratia marcescens strain S7.1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: F0335_RS15830
• Protein ID: WP_149559135.1
• Coordinates: 3298593..3298946 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A177IM12
• Locus tag: F0335_RS15825
• Protein ID: WP_049200011.1
• Coordinates: 3298286..3298588 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F0335_RS15805	3293653..3294588	-	936	WP_149559139.1	alpha/beta hydrolase	-
F0335_RS15810	3294588..3295508	-	921	WP_149559138.1	alpha/beta hydrolase	-
F0335_RS15815	3295647..3296555	+	909	WP_149559137.1	LysR family transcriptional regulator	-
F0335_RS15820	3296667..3297875	-	1209	WP_149559136.1	aldose 1-epimerase family protein	-
F0335_RS15825	3298286..3298588	-	303	WP_049200011.1	XRE family transcriptional regulator	Antitoxin
F0335_RS15830	3298593..3298946	-	354	WP_149559135.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F0335_RS15835	3299384..3300679	+	1296	WP_063989266.1	MFS transporter	-
F0335_RS15840	3300707..3301513	+	807	WP_107226504.1	substrate-binding domain-containing protein	-
F0335_RS15845	3301488..3302387	-	900	WP_149559134.1	LysR family transcriptional regulator	-
F0335_RS15850	3302480..3302845	-	366	WP_060439472.1	diacylglycerol kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13473.35 Da
• Isoelectric Point: 7.7808

>T156866 WP_149559135.1 NZ_CP053572:c3298946-3298593 [Serratia marcescens]
VWEIKTTDVFDDWFSSLNDADRAGVLAALMVLREKGSGLPRPYADTVKGSRYSNMKELRIQSRGEPLRAFFAFDPYRIGI
VLCAGNKAGNEKRFYDRMLQIADREFTCWLNTLNEKE

Nucleotide

• Length: 354 bp

>T156866 NZ_CP069072:1960219-1960467 [Mycobacterium tuberculosis]
ATGGTGATCGATACCTCTGCGCTGGTCGCGATGCTCAACGATGAACCCGAGGCGCAACGGTTCGAGATAGCCGTGGCAGC
AGACCACGTTTGGCTGATGTCGACGGCGTCATATCCGGAGATGGCGACCGTGATCGAAACACGCTTCGGGGAACCGGGGG
GACGTGAACCCAAGGTCAGCGGCCAGCCTCTCCTCTATAAGGGTGACGATTTCGCATGTATCGATATTCGCGCGGTTCTC
GCCGGCTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11017.82 Da
• Isoelectric Point: 5.7316

>AT156866 WP_049200011.1 NZ_CP053572:c3298588-3298286 [Serratia marcescens]
MGRTLEQLIADEKPEVVANAQALAADIMLNIHLAELREKVQKTQVEMAQALGIRQPTVAVMEKPGRDLKLSSLKRYVEAA
GGKLRLDVELPDGSHYEVVL

Nucleotide

• Length: 303 bp

>AT156866 NZ_CP069072:1960007-1960219 [Mycobacterium tuberculosis]
ATGGAATTGGCGGCTCGAATGGGCGAGACTTTGACACAAGCGGTCGTAGTTGCAGTGCGGGAGCAACTGGCCCGCCGGAC
CGGGCGCACCAGATCCATTTCGCTACGCGAGGAGTTGGCCGCCATTGGCCGGCGCTGCGCGGCCTTACCGGTGCTCGACA
CCCGAGCCGCGGACACGATTCTCGGCTACGACGAGCGCGGGTTGCCCGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A177IM12