Detailed information of TA system TA156836

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 741797..742399
• Replicon: chromosome
• Accession: NZ_CP053568
• Organism: Enterobacter cloacae strain MY490

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5E1AN02
• Locus tag: HPB40_RS03630
• Protein ID: WP_013099360.1
• Coordinates: 742088..742399 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HPB40_RS03625
• Protein ID: WP_013099359.1
• Coordinates: 741797..742087 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPB40_RS03610 (HPB40_03585)	739294..740196	+	903	WP_013099356.1	formate dehydrogenase subunit beta	-
HPB40_RS03615 (HPB40_03590)	740193..740828	+	636	WP_013099357.1	formate dehydrogenase cytochrome b556 subunit	-
HPB40_RS03620 (HPB40_03595)	740825..741754	+	930	WP_029882351.1	formate dehydrogenase accessory protein FdhE	-
HPB40_RS03625 (HPB40_03600)	741797..742087	-	291	WP_013099359.1	helix-turn-helix domain-containing protein	Antitoxin
HPB40_RS03630 (HPB40_03605)	742088..742399	-	312	WP_013099360.1	hypothetical protein	Toxin
HPB40_RS03635 (HPB40_03610)	742629..743546	+	918	WP_013099361.1	alpha/beta hydrolase	-
HPB40_RS03640 (HPB40_03615)	743559..744500	-	942	WP_013099362.1	fatty acid biosynthesis protein FabY	-
HPB40_RS03645 (HPB40_03620)	744545..744982	-	438	WP_013099363.1	D-aminoacyl-tRNA deacylase	-
HPB40_RS03650 (HPB40_03625)	744979..745860	-	882	WP_013099364.1	virulence factor BrkB family protein	-
HPB40_RS03655 (HPB40_03630)	745854..746453	-	600	WP_013099365.1	glucose-1-phosphatase	-
HPB40_RS03660 (HPB40_03635)	746572..747372	-	801	WP_013099366.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12210.18 Da
• Isoelectric Point: 9.7550

>T156836 WP_013099360.1 NZ_CP053568:c742399-742088 [Enterobacter cloacae]
MLFIETDIFTEDVKTLLDDDEYHKLQVFLATQPDYGDLIQNTGGLRKIRWLSGGRGKRGGVRVIYFHRTHEFEIRLLLIY
RKGIKDDLSAREKAILKKMIERW

Nucleotide

• Length: 312 bp

>T156836 NZ_CP069071:3817746-3818156 [Mycobacterium tuberculosis]
GTGATCTATATGGACACCTCGGCCCTGACTAAGCTGCTCATCTCCGAGCCCGAGACGACCGAACTGCGGACATGGCTGAC
CGCGCAAAGCGGCCAGGGCGAGGACGCGGCGACAAGCACCCTTGGCCGGGTCGAGTTGATGAGAGTCGTTGCCCGATACG
GACAACCAGGCCAAACTGAGCGTGCGCGTTACCTACTCGACGGGCTCGACATCCTCCCGCTCACCGAACCGGTGATCGGT
CTAGCTGAAACGATCGGACCGGCCACCCTACGTTCTCTCGACGCGATTCACCTCGCGGCCGCAGCCCAGATCAAGCGGGA
ACTGACAGCCTTCGTCACCTACGACCACCGATTGTTGAGCGGATGCCGTGAGGTCGGCTTCGTCACCGCCTCACCCGGCG
CAGTCCGGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10623.13 Da
• Isoelectric Point: 10.4747

>AT156836 WP_013099359.1 NZ_CP053568:c742087-741797 [Enterobacter cloacae]
MDKALFERLTQSIAQMDDIVGGKREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVISALSD

Nucleotide

• Length: 291 bp

>AT156836 NZ_CP069071:3817471-3817749 [Mycobacterium tuberculosis]
GTGGAGGCAATCGGAATCCGAGAACTAAGACAGCACGCATCGCGATACCTCGCCCGGGTTGAAGCCGGCGAGGAACTTGG
CGTCACCAACAAAGGAAGACTTGTGGCCCGACTCATCCCGGTGCAGGCCGCGGAGCGTTCTCGCGAAGCCCTGATTGAAT
CAGGTGTCCTGATTCCGGCTCGTCGTCCACAAAACCTTCTCGACGTCACCGCCGAACCGGCGCGCGGCCGCAAGCGCACC
CTGTCCGATGTTCTCAACGAAATGCGCGACGAGCAGTGA

Structures

Toxin

• AlphaFold DB: A0A5E1AN02