Detailed information of TA system TA156748

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3600047..3600615
• Replicon: chromosome
• Accession: NZ_CP053390
• Organism: Pseudomonas aeruginosa strain TL1285

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: HMJ40_RS16800
• Protein ID: WP_003113526.1
• Coordinates: 3600047..3600325 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HMJ40_RS16805
• Protein ID: WP_216786977.1
• Coordinates: 3600337..3600615 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HMJ40_RS16785 (HMJ40_16800)	3595473..3596711	+	1239	WP_003111578.1	C69 family dipeptidase	-
HMJ40_RS16790 (HMJ40_16805)	3596773..3597420	+	648	WP_003095021.1	carbonate dehydratase	-
HMJ40_RS16795 (HMJ40_16810)	3597490..3599718	-	2229	WP_021264080.1	TonB-dependent receptor	-
HMJ40_RS30800	3599866..3599994	+	129	Protein_3318	integrase	-
HMJ40_RS16800 (HMJ40_16815)	3600047..3600325	+	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HMJ40_RS16805 (HMJ40_16820)	3600337..3600615	+	279	WP_216786977.1	HigA family addiction module antitoxin	Antitoxin
HMJ40_RS16810 (HMJ40_16825)	3601051..3601380	+	330	WP_124142480.1	hypothetical protein	-
HMJ40_RS16815 (HMJ40_16830)	3601373..3601855	+	483	WP_216786978.1	hypothetical protein	-
HMJ40_RS16825 (HMJ40_16840)	3602417..3603397	-	981	WP_157740141.1	hypothetical protein	-
HMJ40_RS16830 (HMJ40_16845)	3603397..3604194	-	798	WP_094069643.1	hypothetical protein	-
HMJ40_RS16835 (HMJ40_16850)	3604195..3605202	-	1008	WP_216786980.1	thymidylate synthase	-
HMJ40_RS16840 (HMJ40_16855)	3605239..3605571	-	333	WP_232519586.1	DUF4031 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T156748 WP_003113526.1 NZ_CP053390:3600047-3600325 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T156748 NZ_CP069070:c1800454-1800077 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10356.84 Da
• Isoelectric Point: 5.8125

>AT156748 WP_216786977.1 NZ_CP053390:3600337-3600615 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
RGVCGEWRADRT

Nucleotide

• Length: 279 bp

>AT156748 NZ_CP069070:c1800708-1800451 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: V6ALY3